›› 2006, Vol. 49 ›› Issue (3): 363-366.

• RESEARCH PAPERS • Previous Articles     Next Articles

cDNA cloning, sequence analysis and tissue expression of a prophenoloxidase gene in Helicoverpa armigera

ZHANG Xiao-Yu , XU Xiao-Yu, ZHANG Jun-Yan,WANG Guo-Xiu,LIU Xu-Sheng   

  1. College of Life Sciences, Central China Normal University
  • Online:2006-07-10 Published:2006-06-20

Abstract:

The cDNA of Helicoverpa armigera prophenoloxidase (PPO) was cloned by means of RT-PCR and RACE. The cDNA was 2 405 bp in length and contained an open reading frame (ORF) of 2 097 bp which encoded 698 amino acid residues. The deduced amino acid sequence showed a high identity to the reported sequence of PPO2 from other insects and shared the typical structural features of PPO from other insects. The results of RT-PCR showed that PPO mRNA was expressed in integument,haemocyte and midgut of H. armigera.
 

Key words: Helicoverpa armigera, prophenoloxidase gene, clone, sequence analysis, tissue expression