Abstract: In order to clarify the unresolved phylogentic relationships of some lineages of the family Satyridae, the fragments of the mitochondrial COⅠ and Cytb genes of 21 Chinese species of the subfamily Elymninae and Satyrinae (Satyridae) were newly amplified and sequenced; meanwhile, the homologous sequences of two foreign species were obtained from the GenBank. Based on these data, the sequence variation and the phylogenetic relationship of these groups were analyzed. The results of the sequence analysis showed that the two combined genes is 1 056 bp in length by alignment, including 648 conserved, 408 variable and 316 parsimonious-informative sites; the average percentage of A+T is 70.8%, which is much higher than that of C+G (29.2%). Using two nymphalid species as the outgroups, their phylogenetic trees were reconstructed with neighbor-joining (NJ), maximum-parsimony (MP) and bayesian inference (BI) methods. The results indicated that the subfamilies Satyrinae, Elymninae and the tribe Lethini all are not monophyletic; the tribes Mycalesini and Lethini should be moved from the subfamily Elymninae to the subfamily Satyrinae; the tribes Satyrini, Melanargiini and Maniolini might have a closest common ancestor; and additionally, the tribes Palaeonymphini, Mycalesini and Ypthimini are also closely related to one another.

Key words: Lepidoptera, Satyridae, COⅠ gene, Cytb gene, molecular phylogentic tree, phylogeny