›› 2009, Vol. 52 ›› Issue (3): 246-253.

• RESEARCH PAPERS • Previous Articles     Next Articles

Cloning of silkworm hsp20.4 promoter and effects of EGT expression driven by the promoter on pupal development in the silkworm, Bombyx mori


  • Online:2009-04-14 Published:2009-03-20

Abstract: In order to verify the activity of the heat shock protein gene expression driven by the promoter of Bombyx mori hsp20.4 gene and the effect of Bombyx mori nuclearpolyhedrosisvirus (BmNPV) egt gene products on the development of silkworm, we amplified hsp20.4 promoter and egt fragments by PCR, and then recombined hsp20.4 promoter with red fluorescent protein gene DsRed and BmNPV egt respectively to form two different recombinant vectors. The former transferred into silkworm BmN cells and tissues showed the transient expression of DsRed, indicating the hsp20.4 promoter sequence possess heat shock protein gene promoter activity in silkworm, while the latter injected into silkworm pupa could delay the pupal development of silkworm by heat induction.

Key words: Bombyx mori, hsp20.4 promoter, Bombyx mori nuclearpolyhedrosisvirus, egt, development