Abstract: 【Aim】In order to provide the important scientific basis for building effective prevention and control system of aquatic leaf beetle Donacia provostii Fairmaire, the diurnal rhythm of adult behaviors were studied. 【Methods】The adults were observed for 5 consecutive days and recorded in a 30 min interval by single rearing separately and rearing in pairs outside, and data was collected and analyzed. All behaviors were divided into six types: resting without contacting water (ROCW), resting with contacting water (RWCW), foraging (FG), moving (MG), mating (MT) and spawning (SG). 【Results】These results showed the adult behaviors of D. provostii were significantly different at various time points or in various time periods (P<0.001). The proportions of four behaviors were significantly different in different spatial positions (P<0.001), and the behaviors on leaf of Nymphoides peltatum (Gmel.) Kuntze or water surface were also significantly different at different time points (P<0.001). 【Conclusion】Both temporal and spatial variation could significantly influence the behaviors of the waterleaf beetle, and the effect of spatial variation is more significant than that of the temporal variation.

Key words: Donacia provostii, adult, behavior, diurnal rhythm;Nymphoides peltatum