Acta Entomologica Sinica ›› 2016, Vol. 59 ›› Issue (2): 200-208.doi: 10.16380/j.kcxb.2016.02.010

• RESEARCH PAPERS • Previous Articles     Next Articles

Analysis of the bacterial diversity in the intestine of larval Procecidochares utilis (Diptera: Trypetidae) based on 16S rDNA gene sequence

ZHANG Mou1, YANG Pu2, ZHU Jia-Ying3, YUAN Yuan1, GUI Fu-Rong1, GAO Xi1,*, WU Guo-Xing1,*   

  1. (1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China; 3. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China)
  • Online:2016-02-20 Published:2016-02-20

Abstract: 【Aim】 To clarify the bacterial diversity in the intestine of larval  Procecidochares utilis. 【Methods】 The V6 regions of the 16S rDNA genes of intestinal bacteria in  P. utilis larvae was sequenced by Illumina HiSeq techniques. The numbers of sequences and operational taxonomic units (OTUs), species abundance and alpha diversity in samples were analyzed using USEARCH and QIIME softwares. 【Results】 A total of 1 593 506 reads were obtained, which were combined to 1 579 372 tags. After filtration, 1 572 860 tags were clustered into 1 341 OTUs. They were annotated into 13 phyla, 4 classes, 6 orders, 7 families, 10 genera, and 4 species. The Proteobacteria was the most dominant, accounting for 99%. At the genus level, Wolbachia was the dominant bacteria(45%). 【Conclusion】 The results show that the bacteria in the intestine of larval P. utilis are diverse. The information of species richness will lay a foundation for further research on the association of the intestinal bacteria with host plant to reveal feeding specialization in P. utilis.

Key words: Procecidochares utilis, intestinal bacteria, diversity, 16S rDNA, Illumina