Abstract: 【Aim】 This study aims to elucidate the locomotive behavior of adults of the 28-spotted potato ladybird, Henosepilachna vigintioctopunctata (Fabricius) moving on ceiling substrata, so as to provide references for pneumatic control technology to be developed and used in prevention and control of agricultural insect pests in China. 【Methods】 Based on the observation with a Sony camera the locomotive behavior parameters of adult H. vigintioctopunctata moving on ceiling substrata were determined, and the relationship between velocity and stride length, stride frequency and duty factor was analyzed, respectively. 【Results】 The results showed that H. vigintioctopunctata adults moved at a speed of 3.77-19.32 mm/s, with a stride length of 2.63-4.51 mm, and a stride frequency of 1.32-4.74 Hz on ceiling substrata. The stride length and stride frequency were both significantly affected by speed and increased with increasing velocity, but stride length changed less than stride frequency. The duty factors of each leg were significantly affected by speed and decreased with increasing velocity. In one stride period, the time of the transfer phase was much less than that of the support phase. 【Conclusion】 H. vigintioctopunctata adults increase their velocity largely by increasing their stride frequency, compared with the effects of stride length.

Key words: Henosepilachna vigintioctopunctata, mechanics of motion, cyclone insect collector, ceiling substrata, locomotive behavior, gait