Abstract: 【Aim】 This study aims to explore the crucial associated genes and metabolic pathways that regulate the diapause of Exorista civilis, and to provide a theoretical basis for clarifying the molecular mechanism of diapause of this species at the transcriptome level. 【Methods】 The Illumina HiSeq^TM 2000 platform was used to perform transcriptome sequencing and bioinformatics analysis of the non-diapause and diapause pupae of E. civilis. KAAS (KEGG Automatic Annotation Server) online pathway comparison tool was used to analyze the KEGG pathway enrichment of the differentially expressed genes that meet the conditions of padj<0.05 and fold change≥32 or padj<0.05 and fold change≤1/32. 【Results】 According to the sequencing results, a total of 58 050 unigenes were obtained. There were 454 diapause-associated genes (DAGs) with the difference of more than 32-fold changes. Among them, 406 DAGs were up-regulated and involved in a total of 134 pathways, including oxidative phosphorylation, citrate cycle and other important pathways, and 48 DAGs were down-regulated and related to 32 pathways. KEGG enriched pathways revealed that the DAGs are mainly involved in signal transduction, endocrine system and carbohydrate metabolism. 【Conclusion】 The transcriptome data of E. civilis obtained in this study have revealed the important metabolic pathways and associated genes in diapause regulation in E.civilis.

Key words: Exorista civilis, transcriptome sequencing, diapause, diapause-associated genes, KEGG analysis