Abstract: 【Aim】 To determine the parasitism and development performance of bisexual and thelytokous lines of Trichogramma dendrolimi on eggs of different strains of Antheraea pernyi, so as to provide the basis for the mass-rearing of richogrammatid parasitoids using A. pernyi eggs as the alternative host. 【Methods】 The biological parameters including parasitism rate, brood size of offspring (number of offspring wasps emerged from a single brood), and proportion of female offspring of bisexual and thelytokous lines of T. dendrolimi on eggs of six strains of A. pernyi ［Kangda (KD), Dasi (DS), Gaoxin (GX), 988 (NEE), Qingda (QD), and Teda (TD)］ were detected, and the quality parameters of eggs of the six strains of A. pernyi (wet weight per egg, protein content, total carbohydrate content and triglyceride content) were determined. The correlation between the quality parameters of A. pernyi eggs and the biological parameters of T. dendrolimi was detected by principal component analysis. 【Results】 The body size of offspring of the bisexual wasps of T. dendrolimi developed on eggs of the NEE strain of A. pernyi were significantly higher than those developed on eggs of the other strains of A. pernyi except for the KD and QD strains. The parasitism rate, brood size of offspring, and proportion of female offspring of the bisexual wasps of T. dendrolimi were not significantly influenced by the strain of A. pernyi. The brood size of offspring of thelytokous wasps of T. dendrolimi developed on eggs of the KD strain of A. pernyi was the highest, being significantly higher than those developed on eggs of the NEE and QD strains of A. pernyi, but without significant difference with those developed on eggs of the DS, GX, and TD strains of A. pernyi. The parasitism rate and brood size of offspring of the thelytokous wasps of T. dendrolimi were not significantly influenced by the strain of A. pernyi. The protein content in eggs of the TD strain of A. pernyi was the highest, significantly higher than those in eggs of the other strains of A. pernyi except for the GX strain. The total carbohydrate content in eggs of the KD strain of A. pernyi and the triglyceride content in eggs of the QD strain of A. pernyi were the highest, significantly higher than those in eggs of the other strains, respectively. The principal component analysis showed that the parasitism rate of bisexual wasps of T. dendrolimi, and the protein content and triglyceride content in A. pernyi eggs were negatively correlated with the total carbohydrate content in A. pernyi eggs. The body size of offspring of the bisexual wasps of T. dendrolimi was negatively correlated with the wet weight per egg of A. pernyi. The parasitism rate of the thelytokous wasps of T. dendrolimi and the triglyceride content in A. pernyi eggs were negatively correlated with the brood size of offspring of T. dendrolimi, the total carbohydrate content, and the wet weight per egg of A. pernyi. The body size of offspring of thelytokous wasps of T. dendrolimi and the total carbohydrate content in A. pernyi eggs were negatively correlated with the protein content in A. pernyi eggs. 【Conclusion】 This study determined the suitable strain of A. pernyi eggs for the mass-rearing procedure of bisexual and thelytokous lines of T. dendrolimi and revealed the relationship between the nutritive indexes of A. pernyi eggs and the biological parameters of T. dendrolimi. The results provide basic data for improving mass-rearing of T. dendrolimi.

Key words: Trichogramma dendrolimi, alternative host, Antheraea pernyi eggs, mass-rearing; thelytokous parthenogernesis