Acta Entomologica Sinica ›› 2021, Vol. 64 ›› Issue (3): 281-301.doi: 10.16380/j.kcxb.2021.03.001

• RESEARCH PAPERS •     Next Articles

Identification of salivary gland proteins of nymphs and male and female adults of Sogatella furcifera (Hemiptera: Delphacidae)

LI Fei, YI Jin-Yu, LIU Yu-Di*, HOU Mao-Lin   

  1.  (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Online:2021-03-20 Published:2021-04-20


 【Aim】 To identify and functionally annotate the salivary gland proteins of Sogatella furcifera and to explore their differences and correlation between different developmental stages and sexes. 【Methods】 Proteins were extracted from salivary gland tissues dissected from anesthetized nymphs, and male and female adults of S. furcifera, subjected to reductive alkylation and enzymolysis, and identified by liquid chromatography tandem mass spectrometry. Then, the salivary gland proteins were functionally annotated and classified by comparison with the Unigene protein database and KOG analysis. 【Results】There were 385, 168 and 82 salivary gland proteins specifically associated with nymphs, and male and female adults of S. furcifera, respectively. In total 319 salivary gland proteins were shared by nymphs and male adults, 60 by nymphs and female adults, and 60 by male and female adults. The KOG functional annotation showed the highest number of proteins associated with cellular processes and signaling, with 81, 22, and 70 proteins of this type specifically associated with nymphs, male adults, and female adults, respectively, and 19, 21 and 12 proteins of this type shared by nymphs and male adults, nymphs and female adults, and male and female adults, respectively, and these proteins are principally involved in post-translational modification, protein turnover, chaperones, intracellular trafficking, secretion and vesicular transport, and signal transduction.【Conclusion】 The salivary gland proteins of S. furcifera are quite active in signal transduction, which may be connected to its piercing and sucking damage to host plants.

Key words: Sogatella furcifera; salivary gland protein, protein function, developmental stage, sex, KOG function annotation, MS analysis