Acta Entomologica Sinica ›› 2022, Vol. 65 ›› Issue (2): 218-227.doi: 10.16380/j.kcxb.2022.02.010

• RESEARCH PAPERS • Previous Articles     Next Articles

Supplementary non-host food sources significantly enhance the biocontrol potential of the thelytokous strain of Diglyphus wani (Hymenoptera: Eulophidae) against Liriomyza sativae (Diptera: Agromyzidae)

HE Jing1,2,#, YE Fu-Yu2,#, DU Su-Jie2, CHENG Xin-Fei2, PAN Li-Ting2, GUO Jian-Yang2, WANG Fu-Lian1,*, LIU Wan-Xue2,*   

  1.  (1. Forewarning and Management of Agricultural and Forestry Pests, Hubei Engineering Technology Center, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Online:2022-02-20 Published:2022-03-10

Abstract: 【Aim】Diglyphus wani is regard as an important biological agent of agromyzid leafminers. It could be produced by thelytoky so that all offspring are female. This is valuable for biocontrol applications. As a host-feeding parasitoid, D. wani could feed on both non-host food and host larvae in fields. In this study, the aim is to explore the effects of supplement of non-host food on biocontrol potential of thelytokous D. wani, which could provide an insight into mass rearing and biocontrol of D. wani.【Methods】The thelytokous strain of D. wani was chosen to be our study species. The late 2nd to early 3rd instar larvae of Liriomyza sativae were provided as hosts for D. wani at the constant temperature of 25℃ in the laboratory. Differences of biocontrol potential of D. wani were compared by providing three types of food [only hosts provided (H), hosts complemented with 20% glucose solution (HGS) and hosts complemented with 20% honey solution (HHS), respectively]. 【Results】Compared with treatment of H, supplying HGS and HHS significantly increased the adult longevity of D. wani (2.4 and 2.1 fold, respectively), the number of parasitism events (3.2 and 2.9 fold, respectively), the number of host-feeding events (2.7 and 2.2 fold, respectively), and the total number of host-killing events (2.8 and 2.4 fold, respectively). Supplying HGS significantly increased the number of host-stinging events as compared with supplying H and HHS, respectively. However, there was no significant difference in the number of host-stinging events between supply of H and HHS. Besides, both supply of HGS and HHS significantly increased the daily number of parasitism events and daily total number of host-killing events, however, both treatments did not alter daily number of host-feeding events and daily number of host-stinging events yet. Moreover, no significant difference was found in the total number of host-killing events between supply of HGS and HHS. When D. wani was provided with the three types of food, the average daily total number of host-killing events was more than 17 hosts and the non-reproductive host-killing events (number of hoststinging events and host-feeding events) accounted for the largest proportion. In addition, when D. wani was provided with the three types of food, the number of host-feeding events showed significantly positive correlations with the number of parasitism events, longevity of parasitoid, and total number of host-killing events.  【Conclusion】D. wani can increase its longevity, enlarge its fecundity and further enhance the host-killing ability through host feeding. Supplementary non-host food sources can further prolong the longevity and improve the host-killing ability of D. wani. Therefore, its thelytokous strain is a promising and potential biocontrol agent against L. sativae. This study could provide guidance for the indoor rearing and field release and application of this parasitoid.

Key words: Diglyphus wani; thelytokous strain, host feeding, non-host food sources, biocontrol potential