Abstract: 【Aim】 This study aims to screen the tobacco varieties, seedling raising methods and sources of Myzus persicae suitable for Aphidius gifuensis parasitization, so as to provide a scientific reference for artificial propagation and rejuvenation of A. gifuensis. 【Methods】We investigated the parasitism rate of A. gifuensis adults on M. persicae nymphs and the emergence rate, life span, body size and proportion of females of A. gifuensis adults when M. persicae nymphs were bred with different varieties of flue-cured tobacco (Yunyan 87, K326, CB-1 and Honghuadajinyuan), from different sources (from tobacco region, from nontobacco region and indoor breeding population), and bred with tobacco with different seedling raising methods (soil seedling raising and moist seedling raising) via pot experiments.【Results】The results showed that different tobacco varieties had significant effects on the parasitism rate of A. gifuensis adults on M. persicae nymphs. The parasitism rates of A. gifuensis adults on M. persicae bred with Honghuadajinyuan and CB-1 were 19.00% and 14.00% higher than that on K326, respectively. However, tobacco varieties had no significant effects on the other indices of A. gifuensis. Sources of M. persicae showed significant effects on the parasitism rate of A. gifuensis. The parasitism rates of A. gifuensis adults on M. persicae nymphs from the non-tobacco region and tobacco region were 20.25% and 16.75% higher than that on M. persicae from indoor breeding population, respectively. However, sources of M. persicae showed no significant effects on the other indices of A. gifuensis adults including emergence rate, life span, body size and proportion of females. Different seedling raising methods of tobacco did not have significant effects on the parasitism rate of A. gifuensis adults. 【Conclusion】 The parasitsm rate of A. gifuensis on M. persicae depends on tobacco varieties and M. persicae sources, but is not impacted by seedling raising methods. Therefore, in practical applications, the parasitic ability of A. gifuensis on M. persicae can be improved by adjustments to tobacco varieties and M. persicae sources, and the application effect of artificial reproduction efficiency of A. gifuensis can be enhanced.

Key words: Aphidius gifuensis, Myzus persicae, tobacco varieties, seedling raising methods, parasitic ability