Abstract: As the most diverse group of animals on earth, insects are closely related to human production and activities, making entomological research both theoretically significant and practically valuable. In the past decade, the rapid development of novel methods and technologies has greatly promoted the research progress of entomological research. In this article, we provided a comprehensive overview of the applications of emerging methods and technologies in such fields as entomological morphological identification, molecular mechanism and pest management, focusing on the main contents of this special issue from seven aspects: Micro-computed tomography, RNA interference, gene editing, artificial intelligence-driven intelligent recognition, nanotechnology, regulation of insect-microorganism symbiotes, and olfactory behavior regulation. We also proposed the challenges faced by the large-scale application and sustainable development of these technologies, and prospected the future development trend.

Key words: Entomology, micro-computed tomography, RNA interference, gene editing, intelligent recognition, nanotechnology, regulation of insect-microorganism symbiotes, olfaction-based behaviorally manipulated technology