›› 2018, Vol. 61 ›› Issue (4): 505-510.doi: 10.16380/j.kcxb.2018.04.013

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周叶鸣1,2, 郅军锐1, 张鑫3, 邹晓2, 杨凯3,*    

  1. (1. 贵州大学昆虫研究所, 贵阳 550025; 2. 贵州大学真菌资源研究所, 贵阳 550025; 3. 上海烟草集团有限责任公司, 上海200082)
  • 出版日期:2018-04-20 发布日期:2018-04-20

Identification and pathogenicity test of a new soil-derived fungus strain of Lecanicillium to Ephestia elutella (Lepidoptera: Pyralidae)

ZHOU Ye-Ming1,2, ZHI Jun-Rui1, ZHANG Xin3, ZOU Xiao2, YANG Kai3, *    

  1.  (1. Institute of Entomology, Guizhou University, Guiyang 550025, China; 2. Institute of Fungal Resource, Guizhou University, Guiyang 550025, China; 3. Shanghai Tobacco Group Limited Liability Company, Shanghai 200082, China)
  • Online:2018-04-20 Published:2018-04-20

摘要:  【目的】蜡蚧菌Lecanicillium是自然界一类重要的昆虫病原真菌,为了获取更多的蜡蚧菌作为控虫真菌资源,对从森林中的根际土壤分离得到的一株蜡蚧菌进行鉴定以及与其他几种病原真菌进行烟草粉斑螟Ephestia elutella的致病性对比。【方法】采用形态特征比较和转录间隔区(ITS)序列构建分子系统树对从贵州贵定松林下土壤中分离的菌株进行综合鉴定,采用浸虫法对烟草粉斑螟3龄幼虫进行致病性筛选。【结果】根据形态特征比较和系统发育分析,该真菌为丝枝蜡蚧菌Lecanicillium aphanocladii。在测试菌株中,环链棒束孢Isaria cateinannulata、球孢白僵菌Beauveria bassiana和丝枝蜡蚧菌对烟草粉斑螟3龄幼虫7 d的校正死亡率分别为79.22%,64.92%和77.92%,表现出较好的致死效果。【结论】丝枝蜡蚧菌对烟草粉斑螟幼虫的致死效果较好,可用作仓储内烟草粉斑螟的生物防治资源。

关键词: 蜡蚧菌, 丝枝蜡蚧菌, 烟草粉斑螟, 致病性, 生物防治, 虫生真菌

Abstract: 【Aim】 Lecanicillium is one group of important fungal pathogens. In this study, a strain separated from the rhizosphere soil in the forest was identified and its pathogenicity to Ephestia elutella was compared with those of some other fungal pathogens so as to acquire more Lecanicillium strains as pest control fungal resources. 【Methods】 The fungal colonies isolated from the soil under pine forests in Guiding, Guizhou were identified by comparison of morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The pathogenecity of the test strains to the 3rd instar larvae of E. elutella was determined by the method of immersing insects into the spore suspension. 【Results】 According to the morphological characterization and phylogenetic tree, the fungi was identified as Lecanicillium aphanocladii. Among the test strains, Isaria cateinannulata, Beauveria bassiana and L. aphanocladii were lethal to E. elutella, with the corrected mortality rates of 79.22%, 64.92% and 77.92%, respectively, against the 3rd instar larvae at 7 d after treatment. 【Conclusion】 L. aphanocladii, a known species in China, shows high lethal effect to E. elutella larvae, and can be used for biological control of E. elutella larvae in the warehouse.

Key words: Lecanicillium, Lecanicillium aphanocladii, Ephestia elutella, pathogenicity, biological control, entomogenous fungi