类肠膜魏斯氏菌通过调节蜕皮激素和胰岛素通路促进黑腹果蝇生长发育(英文)

doi:10.16380/j.kcxb.2018.06.006

›› 2018, Vol. 61 ›› Issue (6): 676-685.doi: 10.16380/j.kcxb.2018.06.006

类肠膜魏斯氏菌通过调节蜕皮激素和胰岛素通路促进黑腹果蝇生长发育(英文)

李恩惠¹, 王晓阳³, 张乐宵³, 白芃³, 赵欣¹, 刘威^2,*, 张策^1,*

(1. 山西医科大学生理学系, 太原 030001; 2. 山西医科大学汾阳学院医学检验系, 山西汾阳 032200; 3. 山西医科大学汾阳学院基础医学院, 山西汾阳 032200)

出版日期:2018-06-20 发布日期:2018-06-20

Weissella paramesenteroides facilitates the systemic growth of Drosophila melanogaster by modulating ecdysone and insulin signaling pathways (In English)

LI En-Hui^1, WANG Xiao-Yang³, ZHANG Yue-Xiao³, BAI Peng³, ZHAO Xin¹, LIU Wei^2,*, ZHANG Ce^1,*

(1. Department of Physiology, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, China; 3. Department of Basic Medical, Fenyang College of Shanxi Medical University, Fenyang, Shanxi 032200, China)

Online:2018-06-20 Published:2018-06-20

摘要/Abstract

摘要： 【目的】本研究旨在揭示果蝇乳酸菌类肠膜魏斯氏菌Weisellas paramesenteroides对黑腹果蝇Drosophila melanogaster生长发育的影响。【方法】利用选择性培养基分离黑腹果蝇成虫肠道乳酸菌，通过16S rRNA基因序列比对鉴定菌株。通过统计从卵至蛹化和羽化的时间检测果蝇发育历期,并以幼虫体表面积为指标检测果蝇生长速率。利用qPCR检测产卵后不同时间生长激素信号通路基因（dib, E74B和PTTH）及胰岛素信号通路基因（DILP2, DILP3和InR）的表达。通过葡萄糖氧化酶法检测3龄幼虫血淋巴中的葡萄糖水平。【结果】从黑腹果蝇成虫肠道内分离到类肠膜魏斯氏菌，并可以在果蝇肠道内有效定殖。类肠膜魏斯氏菌通过提高果蝇生长速率缩短果蝇卵至蛹化和羽化的时间。qPCR结果显示，类肠膜魏斯氏菌增加了dib, E74B和PTTH的表达量，同时增加了DILP2和DILP3的表达量，降低了InR表达量和幼虫血淋巴中的葡萄糖水平。【结论】类肠膜魏斯氏菌是黑腹果蝇的一种共生菌，通过激活蜕皮激素和胰岛素信号通路，促进生长发育。

关键词: 类肠膜魏斯氏菌, 黑腹果蝇, 生长发育, 蜕皮激素信号通路, 胰岛素信号通路

Abstract: 【Aim】 To investigate the effects of lactic acid bacteria Weisellas paramesenteroides on the growth and development of Drosophila melanogaster. 【Methods】 Bacterium was isolated from the gut of D.melanogaster adults with selective medium and identified by BLAST analysis of 16S rRNA gene. The developmeantal duration of D. melanogaster was quantified by counting the time to puparium formation and adult eclosion, and the growth rate was calculated as the body surface area of larvae. The expression levels of genes involved in hormoral (dib, E74B and PTTH) and insulin signaling（DILP2, DILP3 and InR）of D. melanogaster at different time after egg deposition were assayed by quantitative real-time PCR. The glucose level in the hemolymph of the 3rd instar larvae was measured by glucose oxidase assay. 【Results】 W. paramesenteroides was isoloated and efficiently colonized in the gut of D. melanogaster adults. W. paramesenteroides shortened the time to puparium formation and adult eclosion of D. melanogaster by enhancing the growth rate of flies. W. paramesenteroides increased the expression levels of dib, E74B and PTTH, as well as the expression of DILP2 and DILP3, while decreased the expression levels of InR and the glucose level in larval hemolymph.【Conclusion】 W. paramesenteroides is the symbiotic bacterium of D. melanogaster, and promotes its development by activting the ecdysone and insulin signaling pathways.

Key words: Weissella paramesenteroides, Drosophila melanogaster, development, ecdysone signaling pathway, insulin signaling pathway

李恩惠, 王晓阳, 张乐宵, 白芃, 赵欣, 刘威, 张策. 类肠膜魏斯氏菌通过调节蜕皮激素和胰岛素通路促进黑腹果蝇生长发育(英文)[J]. , 2018, 61(6): 676-685.

LI En-Hui, WANG Xiao-Yang, ZHANG Le-Xiao, BAI Peng, ZHAO Xin, LIU Wei, ZHANG Ce. Weissella paramesenteroides facilitates the systemic growth of Drosophila melanogaster by modulating ecdysone and insulin signaling pathways (In English)[J]. , 2018, 61(6): 676-685.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2018.06.006

http://www.insect.org.cn/CN/Y2018/V61/I6/676

[1]	杨阳, 罗坤, 江超, 吴建伟, 朱贵明. 食物中多不饱和脂肪酸在家蝇幼虫体内的富集与代谢及对其生长的影响[J]. 昆虫学报, 2019, 62(5): 578-585.
[2]	李栋, 李晓维, 马琳, 付开赟, 丁新华, 郭文超, 吕要斌. 温度对番茄潜叶蛾生长发育和繁殖的影响[J]. 昆虫学报, 2019, 62(12): 1417-1426.
[3]	闫琴, 王明亮, 花蕾, 王杰, 张宏雨, 刘宇虹, 白芃, 付慧, 张俊洁, 刘威. 短小乳杆菌的代谢产物对黑腹果蝇产卵选择性的影响[J]. 昆虫学报, 2019, 62(12): 1400-1408.
[4]	李寿银, 陈聪, 李慧, 樊斌琦, 王焱, 郝德君. 取食含不同植物源成分的饲料对香樟齿喙象幼虫生长发育及体内解毒酶活性的影响[J]. 昆虫学报, 2019, 62(11): 1286-1296.
[5]	高露, 李钊, 赫晓媛, 甘颖, 金虹云, 闫琴, 白芃, 刘威. 黑腹果蝇对高盐的产卵避性反应及其生物学意义[J]. 昆虫学报, 2019, 62(1): 73-81.
[6]	周思聪, 陈佳妮, 庞兰, 潘中秋, 时敏, 黄健华, 陈学新. 布拉迪小环腹瘿蜂的生物学特性及其寄生对黑腹果蝇生长发育的影响[J]. , 2018, 61(9): 1040-1046.
[7]	张祥乐, 马俐, 马倩, 李胜, 刘素宁. Ras信号通路通过激活转录因子Myc促进核内复制细胞生长[J]. , 2018, 61(8): 885-894.
[8]	董玮, 孔悦, 马恩波, 张建珍, 张敏, 张徐波. 异位表达Dichaete对果蝇足部发育的影响及其作用机制[J]. , 2018, 61(2): 149-155.
[9]	葛超美, 张家侠, 孙钦玉, 叶涛, 夏先江, 张冉, 丁勇. 灰茶尺蠖对不同茶树品种取食选择与适应性及与茶树叶片营养成分的关系[J]. 昆虫学报, 2018, 61(11): 1300-1309.
[10]	王康, 庞倩, 张文文, 吉挺. 多菌灵亚致死剂量对意大利蜜蜂幼虫生长发育和解毒酶系活性的影响[J]. , 2017, 60(6): 642-649.
[11]	李玉娟, 苏琬真, 胡坤坤, 李鹏程, 刘威, 姚红. 植物乳杆菌促进黑腹果蝇生长发育[J]. , 2017, 60(5): 544-552.
[12]	刘威, 张珂, 李恩惠, 徐皓哲, 张策. 大肠杆菌改变果蝇产卵偏嗜性[J]. 昆虫学报, 2017, 60(2): 173-179.
[13]	王璐璐, 陈利荣, 朱景新, 王子光, 赵梓涵, 李曌芳, 刘威. 黑腹果蝇对丙酸的产卵避性反应及其生物学意义[J]. , 2017, 60(12): 1430-1438.
[14]	张维, 郑雅, 王玉凤. 沃尔巴克氏体感染对黑腹果蝇性腺和早期胚胎DNA 6mA去甲基化酶基因DMAD表达的影响[J]. , 2017, 60(11): 1292-1299.
[15]	梁菲菲, 王振营, 何康来, 张天涛, 白树雄. 幼虫期短时高温暴露对二点委夜蛾存活和繁殖的影响[J]. , 2016, 59(9): 991-996.

类肠膜魏斯氏菌通过调节蜕皮激素和胰岛素通路促进黑腹果蝇生长发育(英文)

Weissella paramesenteroides facilitates the systemic growth of Drosophila melanogaster by modulating ecdysone and insulin signaling pathways (In English)

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10