›› 2018, Vol. 61 ›› Issue (6): 712-720.doi: 10.16380/j.kcxb.2018.06.010

• 研究论文 • 上一篇    下一篇


李梅梅#, 李伯辽#, 仵均祥*, 许向利*   

  1. (西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100)
  • 出版日期:2018-06-20 发布日期:2018-06-20

Screening of microsatellite loci and the genetic diversity analysis of Mythimna separata (Lepidoptera: Noctuidae) populations in China

LI Mei-Mei#, LI Bo-Liao#, WU Jun-Xiang*, XU Xiang-Li*    

  1. (State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China)
  • Online:2018-06-20 Published:2018-06-20

摘要:  【目的】筛选适用于我国粘虫Mythimna separata种群遗传学研究的微卫星位点,从分子水平揭示粘虫种群的遗传多样性。【方法】利用已报道的微卫星标记及本实验室粘虫转录组测序的SSR序列,采用PCR产物荧光标记与自动扫描分型方法,分析各位点在我国河南、陕西、山西3个省份的7个粘虫地理种群200头试虫中的扩增稳定性和多态性。【结果】7个粘虫地理种群有7个位点能稳定扩增且具有较高的多态性。这7个位点等位基因丰富度(Ar)为4.167~12.402,观测杂合度(Ho)平均为0.640,期望杂合度(He)平均为0.752,多态信息含量(PIC)为0.547~0.884;各位点均存在无效等位基因且偏离哈迪温伯格平衡,所有成对位点不存在显著连锁不平衡情况。【结论】从来自河南、陕西、山西的7个不同粘虫地理种群中成功筛选了7个能稳定扩增的SSR位点,且在这7个不同的粘虫地理种群中均具有较高的多态性,可用于我国粘虫种群遗传结构研究。粘虫不同地理种群间基因交流频繁,基因交流阻止了由遗传漂变引起的群体间分化,不同地理种群间遗传分化很低甚至不存在遗传分化。

关键词: 粘虫, 微卫星标记, 扩增稳定性, 遗传多样性, 遗传分化, 基因流

Abstract: 【Aim】 This study aims to acquire microsatellite loci available for studying the population genetics of the oriental armyworm, Mythimna separata in China and to further uncover the genetic diversity of its geographical populations from a molecular perspective. 【Methods】 Based on microsatellite markers which had been previously reported in public and  the simple sequence repeats (SSRs) from our laboratory transcriptome of M. separata, the stability of amplification and polymorphism of these microsatellite loci were analyzed based on 200 individuals sampled in seven geographical populations from Henan, Shaanxi and Shanxi in China. The PCR products of SSRs were labeled fluorescently and scanned automatically. 【Results】 Seven microsatellite loci were amplified stably in samples of seven geographical populations of M. separata and possessed high polymorphism. The allele richness (Ar) of the seven microsatellite loci was between 4.167 and 12.402, the average observed heterozygosity (Ho) and expected heterozygosity (He) were 0.640 and 0.752, respectively, and the polymorphic information content (PIC) ranged from 0.547 to 0.884. The seven loci had null allele and deviated from Hardy.Weinberg equilibrium. No significant linkage disequilibrium existed in pairwise loci. 【Conclusion】 Seven microsatellite loci were successfully screened from seven geographical populations of M. separata from Henan, Shaanxi and Shanxi, and they show high polymorphism among these geographical populations and can be used in genetic structure research of this insect. In addition, frequent gene flow occurs in these populations, which prevents population differentiation caused by genetic drift. Moreover, fairly low even no pairwise population differentiation occurs in these populations.

Key words: Mythimna separata, microsatellite marker, amplifying stability, genetic diversity, genetic differentiation, gene flow