›› 2018, Vol. 61 ›› Issue (8): 923-931.doi: 10.16380/j.kcxb.2018.08.005

• 研究论文 • 上一篇    下一篇

球孢白僵菌侵染的柞蚕蛹体壁和脂肪体组织病理观察

申宇1, 安利佳1, 李文利2,*   

  1. (1. 大连理工大学生命科学与技术学院, 辽宁大连 116024; 2. 大连理工大学生命与医药学院, 辽宁盘锦 124221)
  • 出版日期:2018-08-20 发布日期:2018-08-20

Histopathological observation of the integument and fat body of Antheraea pernyi (Lepidoptera: Saturniidae) pupae infected by Beauveria bassiana

SHEN Yu1, AN Li-Jia1, LI Wen-Li2,*     

  1. (1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China; 2. School of Life and Medicine, Dalian University of Technology, Panjin, Liaoning 124221, China)
  • Online:2018-08-20 Published:2018-08-20

摘要: 【目的】探究球孢白僵菌Beauveria bassiana穿透柞蚕Antheraea pernyi蛹体壁和侵染其脂肪体的过程,进一步明确其对柞蚕蛹的致病机理。【方法】利用扫描电镜技术观察球孢白僵菌穿透柞蚕蛹体壁的病理变化,并运用透射电镜技术观察球孢白僵菌侵染的柞蚕蛹脂肪体的病理变化。【结果】扫描电镜观察结果显示:在球孢白僵菌侵染柞蚕蛹体表过程中,分生孢子主要侵染气门、刚毛根部和头部与胸部的节间膜等部位。分生孢子除了从气门、刺突、头部和胸部的节间膜进入体内以外,也可以直接穿透厚而坚硬的体壁进入体内。透射电镜观察结果显示,脂肪体中,球孢白僵菌首先侵染细胞器(线粒体除外),然后再侵染脂肪滴。被侵染的脂肪滴颜色变浅,表面出现许多孔洞,变形,分裂成小的脂肪滴进而完全被侵蚀。蛹僵硬时,脂肪体细胞完全被破坏,只观察到芽生孢子和菌丝。【结论】柞蚕蛹经体表侵染和注射侵染球孢白僵菌处理后,蛹的体壁和脂肪体两组织都发生了明显的病理变化。

关键词: 球孢白僵菌, 柞蚕, 蛹, 扫描电镜, 透射电镜, 侵染过程

Abstract: 【Aim】 The purpose of this study is to investigate the infection process by Beauveria bassiana in the integument and fat body of Antheraea pernyi pupae so as to further clarify the pathogenesis in A. pernyi pupae infected by B. bassiana. 【Methods】 The morphological changes in the integument and fat body of A. pernyi pupae infected by B. bassiana were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. 【Results】 SEM observation indicated that the infection of B. bassiana in A. pernyi pupae mainly occurred at valves, bristles root and the intersegmental membrane between head and thorax. Besides, conidia penetrated into the body directly from the thick and hard wall of the body. TEM observation indicated that in the fat body, organelles except mitochondria were first infected, and then the fat droplets were eroded. The infected fat droplets became shallow, their surface had many holes and became deformed, and the fat droplets were divided into small fat droplets, indicating that they were completely eroded. When pupae were rigid, fat cells were completely destroyed, leaving only the blastospores and hyphae. 【Conclusion】 The integument and fat body of A. pernyi pupae infected by B. bassiana have obvious histopathological changes.

Key words: Beauveria bassiana, Antheraea pernyi, pupae, scanning electron microscopy (SEM), transmission electron microscopy (TEM), infection process