牧草盲蝽危害对寄主植物体内营养物质含量及保护酶活性的影响

doi:10.16380/j.kcxb.2018.08.011

摘要/Abstract

摘要： 【目的】为了明确牧草盲蝽Lygus pratensis危害对不同寄主体内营养物质及保护酶的影响。【方法】采用分光光度法测定了牧草盲蝽偏好度不同的10种寄主植物(6种偏好寄主，偏好性依次为:灰绿藜Chenopodium glaucum＞马齿苋Portulaca oleracea＞反枝苋Amaranthus retroflexus＞紫花苜蓿Medicago sativa＞木地肤Kochia prostrata＞棉花Gossypium hirsutum; 4种非偏好寄主：碱蓬Suaeda glauca、天蓝苜蓿Medicago lupulina、田旋花Convolvulus arvensis和宽叶独行菜Lepidium latifolium)受其危害后叶片中叶绿素、可溶性糖和氨基酸含量以及保护酶活性。【结果】结果表明，受牧草盲蝽为害后，不同寄主植物叶片叶绿素含量均有所下降，其中反枝苋叶片叶绿素含量下降率最低，仅2.6%；紫花苜蓿叶片叶绿素含量下降率最高，达26.21%。可溶性糖含量变化各异,木地肤叶片可溶性糖含量下降率最高，达24.05%，且与对照(未受害株)间存在显著差异；灰绿藜叶片可溶性糖含量上升68.92%，与对照差异极显著。除反枝苋叶片游离脯氨酸含量下降38.87%，其余寄主叶片中游离脯氨酸含量均呈上升趋势。各寄主植物叶片中蛋白质含量均有所下降，反枝苋中下降率最低，仅2.96%。不同寄主中受害后叶片POD活性均呈增强趋势，马齿苋受害后叶片POD活性增幅最高，达74.23%。受害后寄主叶片CAT活性有增有减，灰绿藜、紫花苜蓿和田旋花叶片CAT活性增高，分别增加45.07%, 30.95%和22.47%；马齿苋与天蓝苜蓿受害后叶片CAT活性下降显著。反枝苋、天蓝苜蓿受害后叶片SOD活性下降，其余寄主叶片中SOD活性上升，但均无显著差异。【结论】寄主受害后，叶片叶绿素含量、游离脯氨酸含量变化与牧草盲蝽偏好性具有较好的相关性，而可溶性糖和蛋白质含量及保护酶活性变化无规律性或无显著差异。

关键词: 牧草盲蝽, 寄主植物, 寄主偏好度, 营养物质, 保护酶

Abstract: 【Aim】 To clarify the effects of Lygus pratensis infestation on the nutrients and protective enzymes of host plants. 【Methods】 The contents of chlorophyll, soluble sugar, amino acids, and protective enzyme activities in the leaves of 10 host plants (six preference hosts with the preference in the descending order as Chenopodium glaucum, Portulaca oleracea, Amaranthus retroflexus, Medicago sativa, Kochia prostrate and Gossypium hirsutum, and four non-preference hosts including Suaeda glauca, Medicago lupulina, Convolvulus arvensis and Lepidium latifolium) infested by L. pratensis were determined, respectively, using biochemical methods. 【Results】 After the host plants were infested by L. pratensis, decreased chlorophyll contents were recorded in leaves of all the tested hosts, with the lowest decrease rate in A. retroflexus (2.6%) and the highest in M. sativa (26.21%). The soluble sugar contents varied a lot after L. pratensis infestation. The soluble sugar content in infested leaves of K. prostrata was significantly declined by 24.05%, while significantly increased by 68.92% in infested leaves of C. glaucum. Except that the free proline content in leaves of A. retroflexus was decreased by 38.87%, those in leaves of all the other host plants were stimulated by L. pratensis infestation. The protein contents in leaves of all the host plants were all decreased after L. pratensis infestation, with the lowest decline by 2.96% in A. reflexus as compared to the control (uninjured plants). The POD activities in leaves of different host plants increased after L. pratensis infestation, with the highest increase rate (74.23%) in P. oleracea. After L. pratensis infestation, the CAT activities in leaves of host plants fluctuated a lot. The CAT activities in infested leaves of C. glaucum, M. sativa and C. arvensis increased by 45.07%, 30.95% and 22.47%, respectively, as compared to the control. However, significantly decreased CAT activities were observed in infested leaves of P. oleracea and M. lupulina. Except that the SOD activities in leaves of A. lividus and M. lupulina infested by L. pratensis decreased, the SOD activities in leaves of all the other host plants were increased but showed no significant difference. 【Conclusion】 After host plants are injured by L. pratensis, the changes in the contents of chlorophyll and free proline in leaves of host plants are well correlated with the preference of L. pratensis to these hosts, while the contents of soluble sugar and protein and the protective enzyme activity have no correlation with the host preference of L. pratensis.

Key words: Lygus pratensis, host plants, host preference, nutrients, protective enzymes

苟长青, 孙鹏, 刘端春, 迪丽努尔·艾麦提, 冯宏祖. 牧草盲蝽危害对寄主植物体内营养物质含量及保护酶活性的影响[J]. , 2018, 61(8): 976-983.

GOU Chang-Qing, SUN Peng, LIU Duan-Chun, DI Li-Nuer·Ai-Mai-Ti, FENG Hong-Zu. Effects of Lygus pratensis (Hemiptera: Miridae) infestation on the nutrient contents and protective enzyme activities in host plants[J]. , 2018, 61(8): 976-983.

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链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2018.08.011

http://www.insect.org.cn/CN/Y2018/V61/I8/976

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牧草盲蝽危害对寄主植物体内营养物质含量及保护酶活性的影响

Effects of Lygus pratensis (Hemiptera: Miridae) infestation on the nutrient contents and protective enzyme activities in host plants

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