昆虫学报 ›› 2020, Vol. 63 ›› Issue (11): 1358-1365.doi: 10.16380/j.kcxb.2020.11.008

• 研究论文 • 上一篇    下一篇

温度对亚洲小车蝗成虫体内呼吸代谢相关酶和抗氧化酶活性的影响

郭娜1, 高书晶1,*, 王宁1, 韩海斌1, 徐林波1, 董瑞文2娜仁满都呼2, 娜布其亚2   

  1. (1. 中国农业科学院草原研究所, 呼和浩特 010010; 2. 乌兰察布盟四子王旗草原站, 内蒙古乌兰察布 011800)
  • 出版日期:2020-11-20 发布日期:2020-12-08

Effects of temperature on the activities of respiratory metabolism-related and antioxidant enzymes in adults of Oedaleus asiaticus (Orthoptera: Acridoidea)

GUO Na1, GAO Shu-Jing1,*, WANG Ning1, HAN Hai-Bin1, XU Lin-Bo1, DONG Rui-Wen2Narenmanduhu2, Nabuqiya2   

  1.  (1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot 010010, China; 2. Dorbod Banner Grassland Station of Ulanqab City, Ulanqab, Inner Mongolia 011800, China)
  • Online:2020-11-20 Published:2020-12-08

摘要: 【目的】阐明亚洲小车蝗Oedaleus asiaticus高温耐受能力,明确温度对亚洲小车蝗成虫体内呼吸代谢相关酶和抗氧化酶活性的影响。【方法】在18~42℃温度范围内,以3℃为间隔设置9个温度处理,将亚洲小车蝗成虫置于光照培养箱黑暗条件下处理4 h,后于室温条件下恢复1 h。采用生化方法测定各处理成虫体内糖类代谢酶3磷酸甘油醛脱氢酶(GAPDH)和3磷酸甘油脱氢酶(GPDH),脂类代谢酶3-羟酰辅酶A脱氢酶(HOAD)和三羧酸循环关键酶柠檬酸合成酶(CS),以及3种抗氧化酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性。【结果】实验所测的亚洲小车蝗雌雄成虫体内7种呼吸代谢相关酶和抗氧化酶活性均随温度升高呈现先增后减的趋势。雌雄成虫GAPDH, GPDH, HOAD和CS活性分别在33, 27, 33和39℃时最高,而SOD, CAT和POD活性均在30℃时最高;所测7种酶活性均在18℃时最低。在大多数测试温度下雌雄成虫酶活性差异显著。不同温度下雌、雄成虫的GAPDH/HOAD活性比值在1.99~3.31之间,说明在实验温度下亚洲小车蝗呼吸代谢主要消耗糖类物质。【结论】在测试的18~42℃温度范围内亚洲小车蝗成虫体内呼吸代谢相关酶和抗氧化酶活性随温度升高呈先增后减的趋势,亚洲小车蝗成虫通过调节呼吸代谢及抗氧化酶活性而适应温度变化。

关键词: 亚洲小车蝗, 温度, 呼吸代谢, 抗氧化酶, 酶活性

Abstract: 【Aim】 To elucidate the high temperature tolerance of Oedaleus asiaticus and the effect of temperature on the activities of respiratory metabolism-related and antioxidant enzymes in O. asiaticus adults. 【Methods】 Adults of O. asiaticus were exposed to nine temperature treatments in the range of 18-42℃ at 3℃ intervals under dark conditions in light incubator for 4 h and then recovered at room temperature for 1 h. The activities of respiratory metabolismrelated enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GPDH), 3-hydroxyacyl-CoA dehydrogenase (HOAD) and citrate synthase (CS), and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the adults of all the treatments were determined by biochemical method. 【Results】 The activities of the seven respiratory metabolismrelated and antioxidant enzymes in O. asiaticus adults assayed in the experiment increased first and then decreased with the temperature increasing. The activities of GAPDH, GPDH, HOAD and CS were the highest at 33, 27, 33 and 39℃, respectively, while those of SOD, CAT and POD were the highest at 30℃. The activities of all the tested seven enzymes were the lowest at 18℃. There was significant difference in the enzyme activities between female and male adults at most tested temperatures. The ratios of GAPDH activity to HOAD activity in female and male adults ranged from 1.99 to 3.31 at different temperatures, suggesting that the main carbohydrates are consumed in the respiratory metabolism of O. asiaticus. 【Conclusion】 In the tested temperature range of 18-42℃, the activities of respiratory metabolism-related enzymes and antioxidant enzymes in O. asiaticus adults first increase and then decrease with the increasing of temperature, and O. asiaticus adults adapt to temperature change by regulating the activities of respiratory metabolism-related and antioxidant enzymes.

Key words: Oedaleus asiaticus; temperature, respiratory metabolism, antioxidant enzyme; enzyme activity