昆虫学报 ›› 2022, Vol. 65 ›› Issue (2): 167-175.doi: 10.16380/j.kcxb.2022.02.005

• 研究论文 • 上一篇    下一篇

中华蜜蜂精巢和卵巢差异表达基因分析

成付平1,2, 袁芳3, 席芳贵3, 秦凯鑫1,2, 胡小芬4, 王子龙1,2,*   

  1. (1. 江西农业大学蜜蜂研究所, 南昌 330045; 2. 江西省蜜蜂生物学与饲养重点实验室, 南昌330045; 3. 江西省养蜂研究所, 南昌330052; 4. 江西农业大学动物科学技术学院, 南昌 330045)
  • 出版日期:2022-02-20 发布日期:2022-03-10

Analysis of differentially expressed genes in testes and ovaries of Apis cerana cerana (Hymenoptera: Apidae)

CHENG Fu-Ping1,2, YUAN Fang3, XI Fang-Gui3, QIN Kai-Xin1,2, HU Xiao-Fen4, WANG Zi-Long1,2,*   

  1.  (1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; 2. Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China; 3. Jiangxi Apiculture Research Institute, Nanchang 330052, China; 4. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China)
  • Online:2022-02-20 Published:2022-03-10

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摘要： 【目的】在蜂群中，雄蜂与蜂王都有着发育完全的性腺，但两者达到性成熟的时间却是不同步的。本研究旨在探究中华蜜蜂Apis cerana cerana雄蜂与蜂王生殖腺的基因表达差异。【方法】利用Illumina测序技术对中华蜜蜂雄蜂精巢与蜂王卵巢转录组差异表达基因(differentially expressed genes, DEGs)进行分析。【结果】从中华蜜蜂精巢与卵巢中共鉴定出5 312个差异表达基因，其中2 668个基因在精巢中表达上调，2 644个基因在卵巢中表达上调。并鉴定了11个与性别决定和精子卵子发生相关的候选基因。这些差异表达基因可以归类到1 458个GO功能类别和132个KEGG通路，其中，有4个GO条目和2个KEGG通路显著富集。【结论】这些结果为研究中华蜜蜂繁殖的分子机制提供了有价值的基因表达信息。

关键词: 中华蜜蜂, 转录组, 精巢, 卵巢, 差异表达基因

Abstract: 【Aim】In honeybee colonies, both drones and queens have fully developed gonads, but they do not reach sexual maturity at the same time. The aim of this study is to explore the gene expression difference of gonads between drones and queens of the Asian honey bee, Apis cerana cerana. 【Methods】Illumina sequencing technology was used to analyze the differentially expressed genes (DEGs) in the transcriptome between testis of drone and ovary of queen of A. cerana cerana. 【Results】We identified 5 312 DEGs between testis and ovary of A. cerana cerana, among which 2 668 and 2 644 genes were up-regulated in testis and ovary, respectively. We also identified 11 candidate genes related to sex determination, spermatogenesis and oogenesis. These DEGs could be classified into 1 458 GO functional classes and 132 KEGG pathways with four GO entries and two KEGG pathways significantly enriched. 【Conclusion】These results provide valuable gene expression information for the study of the molecular mechanism of reproduction in A. cerana cerana.

Key words: Apis cerana cerana, transcriptome, testis, ovary, differentially expressed genes

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