昆虫学报 ›› 2024, Vol. 67 ›› Issue (2): 171-182.doi: 10.16380/j.kcxb.2024.02.003

• 研究论文 • 上一篇    下一篇

粘虫生物胺受体基因的鉴定及组织表达谱分析

陈淑婷, 王凯, 王惠鑫, 谢桂英, 赵新成, 陈文波*   

  1. (河南农业大学植物保护学院, 河南省害虫绿色防控国际联合实验室, 郑州 450046)
  • 出版日期:2024-02-20 发布日期:2024-03-27

Identification and tissue expression profiling of the biogenic amine receptor genes of Mythimna separata (Lepidoptera: Noctuidae)

CHEN Shu-Ting, WANG Kai, WANG Hui-Xin, XIE Gui-Ying, ZHAO Xin-Cheng, CHEN Wen-Bo*   

  1.  (Henan International Laboratory for Green Pest Control, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China)
  • Online:2024-02-20 Published:2024-03-27

摘要: 【目的】基于粘虫Mythimna separata成虫脑转录组数据,明确粘虫体内生物胺受体基因的分子特征及组织表达特性,为粘虫生物胺受体基因的功能研究提供基础。【方法】从粘虫成虫脑转录组数据库中筛选生物胺受体基因序列;基于粘虫基因组数据库,对筛选到的生物胺受体进行基因定位;利用最大似然法对鉴定到的生物胺受体进行系统发育分析;利用qRT-PCR检测鉴定到的生物胺受体基因在粘虫雌雄成虫脑、触角、下唇须、翅、足、胸和腹中的表达量。【结果】从粘虫成虫脑转录组数据库中鉴定出17个生物胺受体基因,包括5个多巴胺受体基因、 5个5-羟色胺受体基因、 5个章鱼胺受体基因和2个酪胺受体基因。这17个生物胺受体基因分布于8条染色体上,其中7个受体基因共定位于22号染色体上。鉴定到的粘虫生物胺受体与斜纹夜蛾Spodoptera litura、家蚕Bombyx mori和烟草天蛾Manduca sexta生物胺受体具有较高的同源性。粘虫多巴胺受体基因均在雌雄成虫脑和触角中高表达,其中 MsepDop2, MsepDop3-1, MsepDop3-2和MsepDopEcR在雌成虫脑中的表达量显著高于在雄成虫脑中的表达量;章鱼胺受体基因MsepOA1和MsepOA2B2在胸部高表达,且在雌成虫胸部中的表达量显著高于雄成虫胸部中的, MsepOA3和MsepOA2B1-3在嗅觉组织触角和下唇须中也具有较高的表达量,并且表达量具有性别差异;酪胺受体基因在脑和触角中具有较高的表达量,其中MsepTA2在雌成虫腹部中的表达量显著高于雄成虫腹部中的; Msep5-HT1基因在脑部中的表达量显著高于在其他组织中的,Msep5-HT2基因在脑、触角和下唇须中都具有较高的表达量。【结论】本研究获得了粘虫17个生物胺受体基因,明确了其在雌雄成虫不同组织中的表达模式,并发现部分生物胺受体基因在粘虫嗅觉和生殖组织中的表达具有性别差异,这为进一步研究粘虫生物胺受体的生理功能提供了基础。

关键词: 粘虫, 生物胺受体, 转录组, 系统发育, 表达谱

Abstract: 【Aim】 To clarify the molecular characteristics and tissue expression properties of the biogenic amine receptor genes in Mythimna separata based on the adult brain transcriptome data of M. separata, so as to provide the basis for the functional research of the biogenic amine receptor genes of M. separata. 【Methods】 The biogenic amine receptor gene sequences were screened from the brain transcriptome database of adult M. separata. Gene mapping of the selected biogenic amine receptor genes was analyzed by mapping cDNA with the M. separata genome database. The phylogenetic analysis of the identified biogenic amine receptors was performed by using maximum likelihood method. The qRT-PCR was used to detect the expression levels of the identified biogenic amine receptor genes in the brain, antenna, labipalp, wing, leg, thorax and abdomen of female and male adults of M. separata.【Results】 A total of 17 biogenic amine receptor genes were identified from the transcriptome database of adult M. separata brain, including five dopamine receptor genes, five 5-hydroxytryptamine receptor genes, five octopamine receptor genes and two tyramine receptor genes. The 17 biogenic amine receptor genes were distributed on eight chromosomes, 7 of them were co-located on the chromosome 22. The identified biogenic amine receptors of M. separata had high homology with the biogenic amine receptors of Spodoptera litura, Bombyx mori and Manduca sexta. The dopamine receptor genes were highly expressed in the brain and antenna of female and male adults of M. separata, and the expression levels of MsepDop2, MsepDop3-1, MsepDop3-2 and MsepDopEcR were significantly higher in the female adult brain than those in the male adult brain. The octopamine receptor genes MsepOA1 and MsepOA2B2 were highly expressed in the thorax, and their expression levels in the thorax of female adults were significantly higher than those in the thorax of male adults. The expression levels of MsepOA3 and MsepOA2B1-3 were also higher in the olfactory tissues antenna and labipalp, with gender differences. The expression levels of tyramine receptor genes were high in the brain and antenna, and the expression level of MsepTA2 in the abdomen of female adults was significantly higher than that in the abdomen of male adults. The expression levels of Msep5-HT1 genes in the brain were significantly higher than those in the other tissues. The Msep5-HT2 genes had high expression levels in the brain, antenna, and labipalp.【Conclusion】 In this study, we obtained 17 biogenic amine receptor genes of M. separata, and clarified their expression profiles in different tissues of female and male adults of M. separata. Gender differences in the expression of some biogenic amine receptor genes in olfactory and reproductive tissues were revealed. This research provides a basis for further exploration on the physiological function of biogenic amine receptors in M. separata.

Key words: Mythimna separata, biogenic amine receptor, transcriptome, phylogeny, expression profile