靛灰蝶和彩斑尾蚬蝶线粒体基因组及基于线粒体基因的凤蝶总科系统发育关系

doi:10.16380/j.kcxb.2023.07.009

昆虫学报 ›› 2023, Vol. 66 ›› Issue (7): 946-958.doi: 10.16380/j.kcxb.2023.07.009

靛灰蝶和彩斑尾蚬蝶线粒体基因组及基于线粒体基因的凤蝶总科系统发育关系

闫振天¹, 罗斯特², 范真槐¹, 陈斌^1,*

(1. 重庆师范大学昆虫与分子生物学研究所, 媒介昆虫重庆市重点实验室, 重庆 401331; 2. 厦门大学生命科学学院, 厦门 361102)

出版日期:2023-07-20 发布日期:2023-08-17

Mitochondrial genomes of Caerulea coeligena (Lepidoptera: Lycaenidae) and Dodona maculosa (Lepidoptera: Riodinidae) and phylogenetic relationships in Papilionoidea based on mitochondrial genes

YAN Zhen-Tian¹, LUO Si-Te², FAN Zhen-Huai¹, CHEN Bin^1,*

(1. Chongqing Key Laboratory of Vector Insects, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China; 2. School of Life Sciences, Xiamen University, Xiamen 361102, China)

Online:2023-07-20 Published:2023-08-17

摘要/Abstract

摘要： 【目的】对靛灰蝶Caerulea coeligena和彩斑尾蚬蝶Dodona maculosa线粒体全基因组进行测序和分析，并基于已知线粒体基因组数据探析凤蝶总科(Papilionoidea)的系统发育关系。【方法】利用Illumina NovaSeq 6000测序平台测定靛灰蝶和彩斑尾蚬蝶线粒体全基因组序列，对其进行拼装和注释，并对其tRNA基因二级结构进行预测；基于NCBI数据库中已报道的凤蝶总科6科48种蝴蝶线粒体基因组13个蛋白编码基因(protein-coding genes, PCGs)核苷酸序列，对凤蝶总科50个种的13个PCGs的选择压力进行分析；利用最大似然法(maximum likelihood, ML)和贝叶斯法(Bayesian inference, BI)分析这50个种及其所属科和亚科的系统发育关系。【结果】靛灰蝶和彩斑尾蚬蝶线粒体全基因组长分别是15 164和15 486 bp (GenBank登录号分别为MZ489120和MZ489121)，其基因组大小、结构和组成、基因排列、核苷酸组成、密码子使用和tRNA结构预测等均与已知近缘种蝴蝶相似；凤蝶总科线粒体基因组进化经历了高水平的纯化选择，13个PCGs非同义替换率和同义替换率比值(K_a/K_s)明显小于1。凤蝶总科重建的系统发育关系为：(凤蝶科(Papilionidae)+弄蝶科(Hesperiidae)+粉蝶科(Pieridae)+(蛱蝶科(Nymphalidae)+(灰蝶科(Lycaenidae)+蚬蝶科(Riodinidae))))。【结论】基于凤蝶总科已报道的48种和本研究新测序两种线粒体基因组重建的系统发育树表明蚬蝶科与灰蝶科为单系且互为姐妹群关系，且与蛱蝶科具有更近的亲缘关系，凤蝶科可能是凤蝶总科中最早进化的类群。本研究为进一步研究蝴蝶的系统进化和完善其分类系统提供了新的分子数据支撑。

关键词: 靛灰蝶, 彩斑尾蚬蝶, 凤蝶总科, 线粒体基因组, 系统发育

Abstract: 【Aim】 To sequence and analyze the complete mitochondrial genomes (mtgenomes) of Caerulea coeligena and Dodona maculosa, and to explore the phylogenetic relationships in Papilionoidea based on the known mitochondrial genome data. 【Methods】 The complete mtgenomes of C. coeligena and D. maculosa were sequenced using Illumina NovaSeq 6000 sequencing platform, assembled and annotated, and the secondary structure of tRNA genes was predicted. Based on the nucleotide sequences of 13 protein-coding genes (PCGs) from the mtgenomes of 48 reported butterfly species belonging to six families in Papilionoidea obtained from the NCBI database, the selective pressures on these 13 PCGs in 50 species were analyzed. The phylogenetic relationships among these 50 species, as well as their respective families and subfamilies, were analyzed using maximum likelihood (ML) and Bayesian inference (BI) methods. 【Results】 The mtgenomes of C. coeligena and D. maculosa (GenBank accession numbers are MZ489120 and MZ 489121, respectively) are 15 164 and 15 486 bp in length, respectively. Their genome size, structure and composition, gene arrangement, nucleotide composition, codon usage, and predicted tRNA structure of mitogenomes of C. coeligena and D. maculosa were similar to those of the known closely related butterfly species. The mtgenomes in Papilionoidea exhibited a high level of purifying selection, with the ratios of nonsynonymous substitution rate to synonymous substitution rate (K_a/K_s) for the 13 PCGs obviously lower than 1. The phylogenetic relationships in Papilionoidea were (Papilionidae+Hesperiidae+Pieridae+(Nymphalidae+ (Lycaenidae+Riodinidae))).【Conclusion】 The reconstructed phylogenetic tree based on the mtgenomes of 48 reported butterfly species in Papilionoidea and two newly sequenced species in this study suggests that Riodinidae and Lycaenidae be monophyletic， and sister each other. The sister group would be closely related to Nymphalidae in phylogenetics. Moreover, Papilionidae may represent one of the earliest lineages in Papilionoidea. This study provides new molecular data to support further research on the systematic evolution and refinement of the classification system for butterflies.

Key words: Caerulea coeligena, Dodona maculosa, Papilionoidea, mitochondrial genome, phylogeny

闫振天, 罗斯特, 范真槐, 陈斌. 靛灰蝶和彩斑尾蚬蝶线粒体基因组及基于线粒体基因的凤蝶总科系统发育关系[J]. 昆虫学报, 2023, 66(7): 946-958.

YAN Zhen-Tian, LUO Si-Te, FAN Zhen-Huai, CHEN Bin. Mitochondrial genomes of Caerulea coeligena (Lepidoptera: Lycaenidae) and Dodona maculosa (Lepidoptera: Riodinidae) and phylogenetic relationships in Papilionoidea based on mitochondrial genes[J]. Acta Entomologica Sinica, 2023, 66(7): 946-958.

[1]	刘慧慧, 李恩杰, 曹亮明, 包青春, 王小林, 辛学兵, 杨忠岐. 元宝枫籽象的重要天敌昆虫——元宝枫刺胫广肩小蜂线粒体全基因组的测定与分析[J]. 昆虫学报, 2023, 66(9): 1233-1245.
[2]	林兴雨, 赵特, 宋南. 梨冠网蝽的线粒体基因组测序及臭虫次目科级水平的系统发育分析[J]. 昆虫学报, 2023, 66(7): 934-945.
[3]	胡莉, 杨琳, 田天, 陈斌. 赭翅臀花金龟线粒体全基因组测序及分析[J]. 昆虫学报, 2023, 66(6): 825-834.
[4]	姚余江, 陈斌, 李廷景. 土蜂科线粒体基因组序列测定和分析[J]. 昆虫学报, 2023, 66(1): 99-107.
[5]	邓鋆, 王刚, 卢聪聪, 张江涛, 黄晓磊. 高桥仁蚧线粒体全基因组测序与分析[J]. 昆虫学报, 2022, 65(4): 451-459.
[6]	李荣荣, 李敏, 孙珊珊, 闫江, 张虎芳, 白明. 红角辉蝽和紫翅果蝽线粒体完整基因组测序及蝽亚科系统发育分析(半翅目: 蝽科)(英文)[J]. 昆虫学报, 2022, 65(10): 1343-1353.
[7]	马婷婷, 林菲, 赵楠, 阮用颖, 谢淑燕, 邹宏达, 陈景益, 房伯平, 黄立飞. 入侵害虫甘薯凹胫跳甲的鉴定及线粒体基因组分析[J]. 昆虫学报, 2022, 65(10): 1354-1366.
[8]	孙佳宁, 任天广, 陈婷, 董文鸽. 基于太平洋甲胁虱裂化线粒体基因组推测甲胁虱属祖先线粒体核型[J]. 昆虫学报, 2022, 65(1): 102-111.
[9]	王建霞, 周怡, 信召哲, 赵丹, 肖金花, 黄大卫. 传粉和非传粉榕小蜂线粒体基因组进化差异(英文) [J]. 昆虫学报, 2021, 64(4): 479-489.
[10]	袁飞敏, 魏琮. 基于线粒体基因组的华蝉族系统发育地位研究(半翅目: 蝉科)(英文)[J]. 昆虫学报, 2021, 64(10): 1205-1217.
[11]	田天, 袁缓, 陈斌. 基于线粒体基因组序列的鞘翅目肉食亚目水生类群系统发育分析[J]. 昆虫学报, 2020, 63(8): 1016-1027.
[12]	王丹阳, 王予彤, 于良斌, 韩海斌, 徐林波, 崔艳伟, 康爱国, 庞红岩. 绿眼赛茧蜂线粒体基因组全序列测定和分析[J]. 昆虫学报, 2020, 63(8): 1028-1038.
[13]	赵亚男, 李朝品. 甜果螨全线粒体基因组测序与分析[J]. 昆虫学报, 2020, 63(3): 354-364.
[14]	王运良, 潘忠琪, 陈可可, 陶瑞松, 苏成勇, 郝家胜, 杨群. 基于线粒体基因组控制区序列的中国冰清绢蝶遗传分化和谱系生物地理研究[J]. 昆虫学报, 2019, 62(4): 475-488.
[15]	黄卫东, 梁馨月, 谢秀凤, 王兴民, 陈晓胜. 基于12S, 16S和28S rRNA基因序列的中国小毛瓢虫属系统发育分析[J]. 昆虫学报, 2019, 62(2): 241-254.

靛灰蝶和彩斑尾蚬蝶线粒体基因组及基于线粒体基因的凤蝶总科系统发育关系

Mitochondrial genomes of Caerulea coeligena (Lepidoptera: Lycaenidae) and Dodona maculosa (Lepidoptera: Riodinidae) and phylogenetic relationships in Papilionoidea based on mitochondrial genes

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0