元宝枫籽象的重要天敌昆虫——元宝枫刺胫广肩小蜂线粒体全基因组的测定与分析

doi:10.16380/j.kcxb.2023.09.010

昆虫学报 ›› 2023, Vol. 66 ›› Issue (9): 1233-1245.doi: 10.16380/j.kcxb.2023.09.010

元宝枫籽象的重要天敌昆虫——元宝枫刺胫广肩小蜂线粒体全基因组的测定与分析

刘慧慧^1,*, 李恩杰¹, 曹亮明², 包青春³, 王小林³, 辛学兵¹, 杨忠岐^2,*

(1. 中国林业科学研究院华北林业实验中心, 北京九龙山暖温带森林国家长期科研基地, 北京102300; 2. 中国林业科学研究院森林生态环境与保护研究所, 国家林业和草原局森林保护学重点实验室, 北京100091; 3. 内蒙古科尔沁右翼中旗五角枫自然保护区管理局, 兴安盟 137400)

出版日期:2023-09-20 发布日期:2023-09-27

Sequencing and analysis of the complete mitochondrial genome of Eurytoma acutibialis (Hymenoptera: Eurytomidae), an important natural enemy insect of Bradybatus sp. (Coleoptera: Curculionidae) in China

LIU Hui-Hui^1,*, LI En-Jie1, CAO Liang-Ming², BAO Qing-Chun³, WANG Xiao-Lin³, XIN Xue-Bing¹, YANG Zhong-Qi^2,*

(1. National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China; 2. Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China; 3. Wujiaofeng Natural Reserve of Horqinyouyizhongqi, Xing′an League 137400, China)

Online:2023-09-20 Published:2023-09-27

摘要/Abstract

摘要： 【目的】元宝枫刺胫广肩小蜂Eurytoma acutibialis Yang, Liu et Cao是我国元宝枫种实Acer truncatum的主要害虫元宝枫籽象Bradybatus sp.的寄生蜂，是调查元宝枫籽象时发现的一个新种，在控制元宝枫籽象上可发挥重要作用。为进一步了解该新寄生蜂，测定和分析元宝枫刺胫广肩小蜂的线粒体全基因组序列，从而为膜翅目(Hymenoptera)小蜂总科(Chalcidoidea)广肩小蜂科(Eurytomidae)昆虫系统分类和发育系统关系等研究提供分子生物学证据。【方法】采用 Illumina HiSeqTM4000测序平台测定元宝枫刺胫广肩小蜂线粒体全基因组序列，并进行拼接、注释和分析。基于膜翅目小蜂总科40种昆虫线粒体的13个蛋白质编码基因(protein-coding genes, PCGs)的核苷酸序列，应用贝叶斯法(Bayesian inference, BI)和最大似然法(maximum likelihood, ML)构建系统发育树，分析元宝枫刺胫广肩小蜂与小蜂总科其他昆虫的系统发育关系。【结果】元宝枫刺胫广肩小蜂线粒体基因组全长15 849 bp，A+T含量为8200%，由37个基因组成，包括13个PCGs、2个 rRNA基因、22个tRNA基因和1个非编码控制区(control region, CR)。13个PCGs中，使用频率由高到低的氨基酸依次为Leu，Ile, Met，Phe和Asn；13个PCGs均以标准的ATN(ATT, ATG和ATA)为起始密码子，并以传统的TAA/TAG为终止密码子。22个tRNA基因中，trnI缺受体臂，trnF缺TΨC臂，trnS1缺 DHU臂，trnS2的 DHU臂仅形成一个简单的环，其他均为典型的三叶草结构。基因发生显著重排，其中基因簇nad2-nad1-cytb-nad6-nad4L-nad4-nad5发生倒置，16个tRNA基因出现重排。系统发育分析的结果高度支持广肩小蜂亚科(Eurytominae)的单系性，并显示元宝枫刺胫广肩小蜂与Eurytoma sp. ZJUH 2016013亲缘关系最近。【结论】首次获得元宝枫刺胫广肩小蜂线粒体全基因组序列；基于线粒体基因组的系统发育分析结果表明元宝枫刺胫广肩小蜂隶属于膜翅目广肩小蜂科广肩小蜂属Eurytoma，支持传统形态学分类结果。

关键词: 广肩小蜂科, 元宝枫刺胫广肩小蜂, 元宝枫籽象, 线粒体基因组, 基因重排

Abstract: 【Aim】 Eurytoma acutibialis Yang, Liu et Cao is a parasitic wasp of Bradybatus sp., a serious seed-eating pest of Acer truncatum in China, and is a new species found during the investigation of Bradybatus sp. and can play an important role in controlling Bradybatus sp. To further understand the new parasitoid, the complete mitochondrial genome of E. acutibialis was sequenced and analyzed so as to provide molecular biological evidence for the systematic taxonomy and phylogenetic relationship of hymenopterous wasps (Hymenoptera: Chalcidoidea: Eurytomidae).【Methods】 The complete mitochondrial genome of E. acutibialis was sequenced on Illumina HiSeq^TM4000 sequencing platform and then assembled, annotated and analyzed. Based on the nucleotide sequences of 13 protein-coding genes (PCGs) from 40 species of hymenopteran insects in the Chalcidoidea, the phylogenetic tree was constructed by Bayesian inference (BI) and maximum likelihood (ML) methods, and the phylogenetic relationship between E. acutibialis and other insects in the Chalcidoidea was analyzed.【Results】The mitochondrial genome of E. acutibialis is 15 849 bp in full-length, with the A+T content of 82.00%, and consists of 37 genes including 13 PCGs, 2 rRNA genes, 22 tRNA genes, and a control region (CR). Among the 13 PCGs, the amino acid usage frequencies from high to low are Leu，Ile, Met， Phe and Asn. All the 13 PCGs initiate with the standard start codons of ATN (ATT, ATG and ATA) and terminate with the typical stop codons TAA/TAG. Among the 22 tRNA genes, trnI lacks receptor arm, trnF lacks TΨC-arm, trnS1 lacks DHU arm and DHU arm of trnS2 forms a simple loop. The remaining tRNA genes have a typical cloverleaf structure. There is a significant gene rearrangement, with an inversion occurring in the nad2-nad1-cytb-nad6-nad4L-nad4-nad5 cluster and rearrangements of 16 tRNA genes. The phylogenetic analysis highly supported the monophyly of the subfamily Eurytomidae, and E. acutibialis was the closest to Eurytoma sp. ZJUH 2016013.【Conclusion】The complete mitochondrial genome sequence of E. acutibialis was firstly obtained, and the phylogenetic analysis based on the mitochondrial genome supported its taxonomic position in the genus Eurytoma in the family Eurytomidae in the order Hymenoptera and proved to be consistent with the morphological taxonomy.

Key words: Eurytomidae; Eurytoma acutibialis; Bradybatus sp., mitochondrial genome, gene rearrangement

刘慧慧, 李恩杰, 曹亮明, 包青春, 王小林, 辛学兵, 杨忠岐. 元宝枫籽象的重要天敌昆虫——元宝枫刺胫广肩小蜂线粒体全基因组的测定与分析[J]. 昆虫学报, 2023, 66(9): 1233-1245.

LIU Hui-Hui, LI En-Jie, CAO Liang-Ming, BAO Qing-Chun, WANG Xiao-Lin, XIN Xue-Bing, YANG Zhong-Qi. Sequencing and analysis of the complete mitochondrial genome of Eurytoma acutibialis (Hymenoptera: Eurytomidae), an important natural enemy insect of Bradybatus sp. (Coleoptera: Curculionidae) in China[J]. Acta Entomologica Sinica, 2023, 66(9): 1233-1245.

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元宝枫籽象的重要天敌昆虫——元宝枫刺胫广肩小蜂线粒体全基因组的测定与分析

Sequencing and analysis of the complete mitochondrial genome of Eurytoma acutibialis (Hymenoptera: Eurytomidae), an important natural enemy insect of Bradybatus sp. (Coleoptera: Curculionidae) in China

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