角倍蚜气味结合蛋白和化学感受蛋白基因的鉴定及表达谱

doi:10.16380/j.kcxb.2023.03.003

摘要/Abstract

摘要： 【目的】通过对角倍蚜Schlechtendalia chinensis气味结合蛋白(odorant-binding proteins, OBPs)和化学感受蛋白(chemosensory proteins, CSPs)基因进行鉴定和表达谱分析，为研究OBPs和CSPs在角倍蚜嗅觉感知中的功能提供理论依据。【方法】基于角倍蚜基因组和不同发育阶段转录组数据，通过生物信息学方法鉴定和筛选角倍蚜OBPs和CSPs的基因序列；利用邻接法对角倍蚜与其他半翅目昆虫OBPs和CSPs的氨基酸序列进行系统发育分析；根据角倍蚜OBP和CSP基因在不同发育阶段(干雌、干母、春迁蚜、秋迁蚜、越冬若蚜、雌性蚜和雄性蚜)转录组数据中的TPM(tags per million)值进行表达丰度分析，结合qRT-PCR方法检测角倍蚜OBP和CSP基因在不同发育阶段的相对表达量。【结果】从角倍蚜基因组和转录组数据中鉴定出12个OBP基因(SchiOBP2-10, SchiOBP13-15)和9个CSP基因(SchiCSP1-2, SchiCSP4-10)；系统发育分析发现，角倍蚜OBPs和CSPs与其他蚜虫OBPs和CSPs聚在一起形成多个同源亚群，具有可信的同源进化关系。不同发育阶段转录组表达丰度和qRT-PCR检测的时空表达谱表明, SchiOBP3/6和SchiCSP1/4/6/9/10在干母中高表达，SchiOBP4/10/14和SchiCSP2/5/7在雄性蚜中高表达，SchiOBP2在干母和雄性蚜中均高表达，SchiOBP7和SchiCSP8在春迁蚜中高表达，SchiOBP8/13/15在秋迁蚜中高表达，SchiOBP9在越冬若蚜中高表达，表明这些基因可能在角倍蚜寻找寄主植物和搜寻配偶的嗅觉识别过程中发挥着重要作用。【结论】本研究鉴定和明确了角倍蚜OBP和CSP基因及其在不同发育阶段的表达特征，为进一步研究角倍蚜嗅觉相关蛋白的功能及瘿蚜与寄主植物的化学通讯机制提供了依据。

关键词: 角倍蚜, 气味结合蛋白, 化学感受蛋白, 系统发育, 表达谱

Abstract:

【Aim】In order to provide theoretical basis for studying the roles of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in olfactory recognition of Schlechtendalia chinensis by the gene identification and expression profiling of OBPs and CSPs of S. chinensis. 【Methods】Based on the genome and transcriptome data of different developmental stages of S. chinensis, the gene sequences of OBPs and CSPs were identified and screened by bioinformatics methods. Phylogenetic analysis of OBPs and CSPs

from S. chinensis and other Hemiptera insects based on amino acid sequences was performed using the neighbor-joining method. According to the TPM (tags per million) values of OBP and CSP genes in the transcriptome of different developmental stages (fundatrigeniae, fundatrix, spring migrant aphids, autumn migrant aphids, overwintering nymphs, sexual females and sexual males) of S. chinensis, the expression abundance was analyzed, and the relative expression levels of OBP and CSP genes were detected by qRT-PCR method. 【Results】 A total of 12 OBP genes (SchiOBP2-10, SchiOBP13-15) and 9 CSP genes (SchiCSP1-2, SchiCSP4-10) were identified from the genome and transcriptome data of S. chinensis. The phylogenetic analysis showed that OBPs/CSPs of S. chinensis were clustered together with OBPs/CSPs of other aphids and formed multiple homologous subgroups, indicating a credible homologous evolutionary relationship. According to the expression abundance in the transcriptome of different developmental stages and spatiotemporal expression profiles detected by qRT-PCR, SchiOBP3/6 and SchiCSP1/4/6/9/10 were highly expressed in fundatrix, SchiOBP4/10/14 and SchiCSP2/5/7 were highly expressed in sexual males, SchiOBP2 was highly expressed in fundatrix and sexual males, SchiOBP7 and SchiCSP8 were highly expressed in spring migrant aphids, SchiOBP8/13/15 were highly expressed in autumn migrant aphids, and SchiOBP9 was highly expressed in overwintering nymphs, indicating that these genes may play
important roles in the olfactory recognition of S. chinensis in searching for host plants and mates.【Conclusion】 In this study, the OBP/CSP genes and their expression characteristics in different developmental stages of S. chinensis were identified and

clarified, which provide a basis for further study on the function of olfactory-related proteins of S. chinensis and the chemical communication mechanism between gall aphids and their host plants.

Key words: Schlechtendalia chinensis, odorant-binding protein, chemosensory protein; phylogeny, expression profile

丰国蕊, 魏洪媛, 许鑫, 邵淑霞, 杨子祥. 角倍蚜气味结合蛋白和化学感受蛋白基因的鉴定及表达谱[J]. 昆虫学报, 2023, 66(3): 292-302.

FENG Guo-Rui, WEI Hong-Yuan, XU Xin, SHAO Shu-Xia, YANG Zi-Xiang. Identification and expression profiling of odorant-binding protein and chemosensory protein genes in Schlechtendalia chinensis (Hemiptera: Aphididae)[J]. Acta Entomologica Sinica, 2023, 66(3): 292-302.

[1]	张凯遥, 张文德, 王紫馨, 胡颖, 钱加珺, 王思懿, 赵浩东, 顾小雨, 牛庆生, 付中民, 陈大福, 郭睿. 东方蜜蜂微孢子虫侵染意大利蜜蜂工蜂过程中nce-miR-10660及其靶基因表达谱[J]. 昆虫学报, 2023, 66(2): 181-189.
[2]	李良斌, 武丽仙, 许雅静, 刘芳, 陈大福, 刘家莉, 赵红霞. 蜂巢小甲虫气味结合蛋白基因AtOBP1的分子特性及功能研究[J]. 昆虫学报, 2023, 66(1): 19-26.
[3]	姚余江, 陈斌, 李廷景. 土蜂科线粒体基因组序列测定和分析[J]. 昆虫学报, 2023, 66(1): 99-107.
[4]	王明明, 殷鹏凯, 李铭晖, 杨自忠, 杨大松, 杨银河. 药用昆虫喙尾琵琶甲肠道抗菌活性放线菌的筛选及鉴定[J]. 昆虫学报, 2022, 65(9): 1106-1114.
[5]	刘盼静, 魏洪义, 郭坤, 马广源, 张涛. 绿芫菁成虫触角中气味结合蛋白和化学感受蛋白基因的鉴定和表达分析[J]. 昆虫学报, 2022, 65(8): 927-936.
[6]	唐浩宇, 刘靖涛, 谢佼昕, 羿超群, 刘晓旭, 张永军, 孙洋. 多异瓢虫气味结合蛋白HvarOBP2的基因克隆及配体结合特性分析[J]. 昆虫学报, 2022, 65(8): 977-985.
[7]	姚玮宸, 徐继伟, 徐鹿, 朱秀云, 张亚楠. 梨木虱化学感受蛋白CchiCSP8与梨树挥发物的结合特性分析[J]. 昆虫学报, 2022, 65(7): 818-830.
[8]	洪波, 常青, 翟颖妍, 任博文, 谢毓芬, 张锋. 枣食芽象甲化学感受蛋白PyasCSP4的基因克隆、表达及配体结合特征[J]. 昆虫学报, 2022, 65(7): 831-842.
[9]	邓鋆, 王刚, 卢聪聪, 张江涛, 黄晓磊. 高桥仁蚧线粒体全基因组测序与分析[J]. 昆虫学报, 2022, 65(4): 451-459.
[10]	刘靖涛, 王倩, 赵瑞, 王欢欢, 高洁, 董辉, 张永军, 丛斌, 谷少华. 豌豆蚜触角转录组化学感受蛋白的鉴定、表达和结合特性分析[J]. 昆虫学报, 2022, 65(3): 261-270.
[11]	刘倩, 罗迪, Roman JASHENKO, 季荣. 意大利蝗海藻糖酶基因克隆及卵低温驯化下的表达模式[J]. 昆虫学报, 2022, 65(2): 157-166.
[12]	李凯旋, 李艳艳, 王树娟, 斯琴, 张丽莹, 庞保平. 桃小食心虫成虫期高表达气味结合蛋白基因的克隆与表达谱分析[J]. 昆虫学报, 2022, 65(11): 1426-1236.
[13]	李荣荣, 李敏, 孙珊珊, 闫江, 张虎芳, 白明. 红角辉蝽和紫翅果蝽线粒体完整基因组测序及蝽亚科系统发育分析(半翅目: 蝽科)(英文)[J]. 昆虫学报, 2022, 65(10): 1343-1353.
[14]	魏艳, 刘勇, 叶倩, 卢丁伊慧, 张战泓, 张卓, 张德咏, 章松柏, 史晓斌. 下调烟粉虱MED隐种BtabCSP6表达对番茄黄化曲叶病毒传播的影响[J]. 昆虫学报, 2022, 65(10): 1287-1294.
[15]	张肖肖, 雷丹, 李香盈, 陈斌. 中华按蚊含LY结构域的胰蛋白酶基因的分子特征及表达模式分析[J]. 昆虫学报, 2021, 64(9): 1050-1060.

角倍蚜气味结合蛋白和化学感受蛋白基因的鉴定及表达谱

Identification and expression profiling of odorant-binding protein and chemosensory protein genes in Schlechtendalia chinensis (Hemiptera: Aphididae)

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0