下调烟粉虱MED隐种BtabCSP6表达对番茄黄化曲叶病毒传播的影响

doi:10.16380/j.kcxb.2022.10.005

昆虫学报 ›› 2022, Vol. 65 ›› Issue (10): 1287-1294.doi: 10.16380/j.kcxb.2022.10.005

下调烟粉虱MED隐种BtabCSP6表达对番茄黄化曲叶病毒传播的影响

魏艳^1,2, 刘勇², 叶倩^1,2, 卢丁伊慧², 张战泓³, 张卓², 张德咏², 章松柏^1,*, 史晓斌^2,*

(1. 长江大学, 农林病虫害预警与调控湖北省工程技术研究中心, 湖北荆州 434025; 2. 湖南省农业科学院植物保护研究所, 长沙 410125; 3. 湖南省农业科学院蔬菜研究所, 长沙 410125)

出版日期:2022-10-20 发布日期:2022-11-27

Influence of down-regulating the expression of BtabCSP6 in Bemisia tabaci MED (Hemiptera: Aleyrodidae) on the transmission of Tomato yellow leaf curl virus

WEI Yan^1,2, LIU Yong², YE Qian^1,2, LU Ding-Yi-Hui², ZHANG Zhan-Hong³, ZHANG Zhuo², ZHANG De-Yong², ZHANG Song-Bai^1,*, SHI Xiao-Bin^2,*

(1. Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, Hubei 434025, China; 2. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 3. Institute of Vegetable, Hunan Academy of Agricultural Sciences, Changsha 410125, China)

Online:2022-10-20 Published:2022-11-27

摘要/Abstract

摘要： 【目的】番茄黄化曲叶病毒(tomato yellow leaf curl virus， TYLCV)是对农业生产造成威胁的主要病毒之一，自然条件下通过媒介昆虫烟粉虱Bemisia tabaci传播。已有研究表明烟粉虱雌成虫比雄成虫具有更强的获毒与传毒能力。本研究旨在探明烟粉虱化学感受蛋白(chemosensory protein, CSP)基因BtabCSP6表达对病毒传播的影响，为控制病毒发生寻找新途径。【方法】使用TYLCV侵染性克隆方法获得带毒番茄植株，微虫笼收集不带毒烟粉虱MED隐种成虫固定在感染TYLCV的番茄植株叶片获毒48 h；利用RT-qPCR技术测定分别取食感染和未感染TYLCV番茄植株的烟粉虱MED隐种雌雄成虫体内BtabCSP1-8基因表达量变化；通过饲喂法利用RNAi对烟粉虱MED隐种雌成虫BtabCSP6基因进行干扰48 h后饲喂TYLCV感染的番茄植株，测定烟粉虱MED隐种雌成虫的获毒率和传毒率。【结果】RT-qPCR检测结果表明，与未侵染的烟粉虱MED隐种雌成虫相比，侵染TYLCV的雌成虫体内BtabCSP3和BtabCSP6基因的表达量变化最为显著。同样地，在侵染TYLCV的雄成虫体内，BtabCSP4和BtabCSP6表达量的变化最为明显。饲喂dsBtabCSP6 48 h后，烟粉虱MED隐种雌成虫体内BtabCSP6表达量降低；取食感染TYLCV番茄植株不同时间烟粉虱MED隐种雌成虫的获毒率和不同数量雌成虫对未感染TYLCV的番茄植株的传毒率与对照相比均显著降低。【结论】下调烟粉虱MED隐种雌成虫体内BtabCSP6基因的表达，可显著降低烟粉虱MED隐种雌成虫的获毒率和传毒率，说明BtabCSP6可能影响TYLCV传播。

关键词: 烟粉虱, 番茄黄化曲叶病毒, 化学感受蛋白, RNA干扰, 基因表达

Abstract: 【Aim】 Tomato yellow leaf curl virus (TYLCV) is one of the main viruses that threaten agricultural production and transmitted by the insect vector Bemisia tabaci under natural conditions. Studies have shown that female adults of B. tabaci have stronger ability to acquire and transmit virus than male adults. The objective of this study is to investigate the influence of the expression of the chemosensory protein (CSP) gene BtabCSP6 in B. tabaci on virus transmission, and to find a new way to control the occurrence of virus. 【Methods】 Infected tomato plants were obtained by the TYLCV infectious clone, and uninfected B. tabaci MED adults were collected in micro-cages and fixed on leaves of the TYLCV-infected tomato plants for 48 h. RT-qPCR technology was used to measure the changes in the expression levels of BtabCSP1-8 in female and male adults of B. tabaci MED fed with TYLCV-infected and uninfected tomato plants, respectively. The BtabCSP6 gene in female adults of B. tabaci MED was suppressed by RNAi for 48 h through feeding method followed by feeding with TYLCV-infected tomato plants, and the virus acquisition and transmission rates of female adults of B. tabaci MED were determined. 【Results】The RT-qPCR results showed that the expression levels of BtabCSP3 and BtabCSP6 in TYLCV-infected female adults of B. tabaci MED changed most significantly as compared to those in the uninfected female adults. Similarly, the expression levels of BtabCSP4 and BtabCSP6 in TYLCV-infected male adults of B. tabaci MED changed most significantly as compared to those in the uninfected male adults. After being fed with dsBtabCSP6 for 48 h, the expression level of BtabCSP6 in female adults of B. tabaci MED decreased. The virus acquisition rates of female adults of B. tabaci MED after feeding with TYLCV-infected tomato plants for different time and the virus transmission rates of female adults of B. tabaci MED in different numbers to uninfected tomato plants were significantly decreased as compared to those of the control. 【Conclusion】 Down-regulating the expression of BtabCSP6 in female adults of B. tabaci MED can significantly reduce their virus acquisition and virus transmission rates, suggesting that BtabCSP6 may affect the transmission of TYLCV.

Key words: Bemisia tabaci; Tomato yellow leaf curl virus, chemosensory proteins, RNA interference, gene expression

魏艳, 刘勇, 叶倩, 卢丁伊慧, 张战泓, 张卓, 张德咏, 章松柏, 史晓斌. 下调烟粉虱MED隐种BtabCSP6表达对番茄黄化曲叶病毒传播的影响[J]. 昆虫学报, 2022, 65(10): 1287-1294.

WEI Yan, LIU Yong, YE Qian, LU Ding-Yi-Hui, ZHANG Zhan-Hong, ZHANG Zhuo, ZHANG De-Yong, ZHANG Song-Bai, SHI Xiao-Bin. Influence of down-regulating the expression of BtabCSP6 in Bemisia tabaci MED (Hemiptera: Aleyrodidae) on the transmission of Tomato yellow leaf curl virus[J]. Acta Entomologica Sinica, 2022, 65(10): 1287-1294.

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下调烟粉虱MED隐种BtabCSP6表达对番茄黄化曲叶病毒传播的影响

Influence of down-regulating the expression of BtabCSP6 in Bemisia tabaci MED (Hemiptera: Aleyrodidae) on the transmission of Tomato yellow leaf curl virus

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