昆虫学报 ›› 2022, Vol. 65 ›› Issue (4): 437-450.doi: 10.16380/j.kcxb.2022.04.004

• 研究论文 • 上一篇    下一篇

重金属复合污染对眼优角蚱代谢组的影响

容万韬1, 宋哲1, 辛磊1, 邓维安2, 覃玉月1, 李晓东1,*   

  1. (1. 河池学院化学与生物工程学院, 广西河池 547000; 2. 广西师范大学生命科学学院, 广西桂林 541000)
  • 出版日期:2022-04-20 发布日期:2022-03-24

Effects of combined pollution of heavy metals on the metabolomics of Eucriotettix oculatus (Orthoptera: Tetrigidae)

RONG Wan-Tao1, SONG Zhe1, XIN Lei1, DENG Wei-An2, QIN Yu-Yue1, LI Xiao-Dong1,*    

  1. (1. College of Chemistry and Bioengineering, Hechi University, Hechi, Guangxi 547000, China; 2. College of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541000, China)
  • Online:2022-04-20 Published:2022-03-24

摘要: 【目的】对金属矿区和非金属矿区环境中生活的眼优角蚱Eucriotettix oculatus体内重金属含量及其代谢组进行分析,探究重金属复合污染对眼优角蚱体内重金属累积和代谢组的影响。【方法】使用ICP-MS法对眼优角蚱成虫体内重金属含量进行测定,同时采用基于 UPLC-MS/MS检测平台、自建数据库以及多元统计分析相结合的手段,对金属矿区和非金属矿区眼优角蚱成虫肠道中的代谢物进行差异分析,并利用 KEGG 数据库对差异代谢物进行注释以及通路富集分析。【结果】金属矿区眼优角蚱成虫体内9种重金属含量是非金属矿区的0.4~212.4倍。多元统计分析结果表明,金属矿区眼优角蚱成虫肠道中共有112种代谢物的含量发生了显著变化,主要为氨基酸类、脂肪酰类、有机酸类、核苷酸类、苯类等物质。KEGG注释及通路富集分析显示,可被注释到的显著差异代谢物共有49种,与代谢通路相关的显著差异代谢物有40种,富集最显著的通路是甲状腺激素合成通路、催产素信号通路、胆汁分泌通路、酪氨酸代谢通路。【结论】重金属复合污染环境中生存的眼优角蚱成虫体内有多类重金属累积,重金属可改变眼优角蚱肠道中的代谢物的组成,其中一些代谢物的改变可能是眼优角蚱适应重金属复合污染生境的策略。

关键词: 眼优角蚱; 重金属, 复合污染; 肠道组织; 代谢组学; 代谢通路

Abstract: 【Aim】 To analyze the contents of heavy metals and metabolomics of Eucriotettix oculatus living in metal and non-metal mining areas, and to explore the effects of combined pollution of heavy metals on the accumulation and metabolomics of heavy metals in E. oculatus. 【Methods】 ICP-MS method was used to determine the content of heavy metals in E. oculatus adults. At the same time, based on UPLC-MS/MS detection platform, selfbuilt database and multivariate statistical analysis, the differences of metabolites in the intestine of E. oculatus adults between the metal mining area and the non-metal mining area were analyzed, and KEGG database was used to conduct annotation and pathway enrichment analysis of the differential metabolites. 【Results】 The contents of nine heavy metals in E. oculatus adults in the metal mining area were 0.4-212.4 times as high as those in the non-metal mining area. The results of multivariate statistical analysis showed that the contents of 112 metabolites changed significantly in the intestine of E. oculatus adults in the metal mining area, mainly including amino acids, fatty acyls, organic acids, nucleotides and benzenes. KEGG annotation and pathway enrichment analysis showed that there were 49 significantly differential metabolites that could be annotated, and 40 significantly differential metabolites were related to metabolic pathways. The most significant enrichment pathways included thyroid hormone synthesis pathway, oxytocin signaling pathway, bile secretion pathway and tyrosine metabolism pathway. 【Conclusion】 There are multiple kinds of heavy metals accumulated in E. oculatus adults living in the environment polluted by multiple heavy metals. Heavy metals can change the composition of metabolites in the intestine of E. oculatus, and the change of some metabolites may be a strategy for E. oculatus to adapt to the habitat polluted by multiple heavy metals.

Key words: Eucriotettix oculatus, heavy metals, combined pollution, intestinal tissue, metabolomics, metabolic pathway