利用植物提取物绿色合成纳米银粒子及其对台湾乳白蚁的毒性

doi:10.16380/j.kcxb.2022.04.008

昆虫学报 ›› 2022, Vol. 65 ›› Issue (4): 480-489.doi: 10.16380/j.kcxb.2022.04.008

利用植物提取物绿色合成纳米银粒子及其对台湾乳白蚁的毒性

谢晓俊¹，羊桂英¹，于保庭²，胡寅²，莫建初^1,*

(1. 浙江大学昆虫科学研究所, 农业农村部农业昆虫学重点实验室, 浙江省作物病虫生物学重点实验室, 杭州 310058; 2. 全国白蚁防治中心, 杭州 310011)

出版日期:2022-04-20 发布日期:2022-03-24

Green synthesis of silver nanoparticles using plant extracts and their toxicity to Coptotermes formosanus (Blattodea: Rhinotermitidae)

XIE Xiao-Jun¹, YANG Gui-Ying¹, YU Bao-Ting², HU Yin², MO Jian-Chu^1,*

(1.Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058; China; 2. National Termite Control Center of China, Hangzhou 310011, China)

Online:2022-04-20 Published:2022-03-24

摘要/Abstract

摘要： 【目的】利用多种药用植物水提液绿色合成纳米银粒子，测定分析其对台湾乳白蚁Coptotermes formosanus的毒杀活性和作用机理，探索绿色合成的(silver nanoparticles, AgNPs)在白蚁防治方面的潜力，拓宽AgNPs在农业领域的应用前景。【方法】分别利用药用植物大黄Rheum palmatum根茎、白毛夏枯草Ajuga nipponensis全株、苦参Sophora flavescens根和鱼腥草Houttuy niacordata全株的水提取液绿色合成纳米银粒子，采用紫外可见分光光度计(UV-vis)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线能谱分析(EDS)和纳米粒度分析仪验证AgNPs的生成并表征其粒子大小、形状和聚集程度等；在实验室条件下测定AgNPs对台湾乳白蚁工蚁的毒杀效果。通过测定800 mg/L AgNPs处理7 d的台湾乳白蚁工蚁体内可溶性蛋白质含量、乙酰胆碱酯酶(AchE)活性和滤纸酶活性(filter paper activity, FPA)水平，来探析AgNPs对白蚁的毒杀作用机理。【结果】4种药用植物水提取液合成的AgNPs颗粒均呈球形，粒径在69~180 nm之间；7 d内AgNPs对台湾乳白蚁工蚁的LC₅₀值分别为150, 340, 342和309 mg/L；800 mg/L AgNPs处理7 d后台湾乳白蚁工蚁的可溶性蛋白质含量以及AchE活性和FPA与对照相比均显著降低。【结论】4种植物提取物合成的AgNPs对台湾乳白蚁工蚁均有较好的毒杀效果，它们可通过降低白蚁体内的可溶性蛋白质含量、AchE活性和FPA来影响其存活，说明绿色合成的AgNPs在防治台湾乳白蚁方面具有较大的潜力。

关键词: 台湾乳白蚁；纳米银粒子, 植物提取液, 绿色合成, 杀虫活性, 作用机制

Abstract: 【Aim】 This study aims to make green synthesis of silver nanoparticles (AgNPs) using aqueous extracts of multiple medicinal plants, and to detect and analyze their toxicity and action mechanism against the Formosan subterranean termite, Coptotermes formosanus, so as to explore the potential of AgNPs in termite control and expand its future application in agriculture. 【Methods】 The aqueous extracts of four medicinal plants including root of rhubarb (Rheum palmatum), plants of decumbent bugle herb (Ajuga nipponensis), root of lightyellow sophorn (Sophora flavescens) and plants of houttuynia (Houttuynia cordata) were used as the raw materials to synthesize AgNPs. The formation of AgNPs was characterized by UV-visiblespectroscopy (UV-vis), scanning electron microscopy (SEM), transmission electronmicroscopy (TEM), X-ray energy dispersive spectrum(EDS) and nanoparticle size analyzer to assess the size, shape and aggregation degree of AgNPs. Then, the toxicity of AgNPs to C. formosanus workers was measured in the laboratory condition and the action mechanism of AgNPs on termites was explored by determining the soluble protein content, acetylcholinesterase (AchE) activity and filter paper activity (FPA) in C. formosanus workers at 7 d after treatment with 800 mg/L AgNPs.【Results】 The green synthesized AgNPs with the aqueous extracts of four medicinal plants were spherical particles, with an average particle diameter of 69-180 nm. The LC₅₀ values of AgNPs against C. formosanus workers in 7 d were 150, 340, 342 and 309 mg/L, respectively. The soluble protein contents, AchE activities and FPA in C. formosanus workers at 7 d after treatment with 800 mg/L AgNPs were significantly decreased compared with those in the control. 【Conclusion】 AgNPs synthesized by four plant extracts show high toxicity to C. formosanus workers, and the survival of termites can be affected by AgNPs by reducing the soluble protein content, AchE activity and FPA, suggesting that AgNPs have great potential in control of C. formosanus.

Key words: Coptotermes formosanus, silver nanoparticles, plant extracts, green synthesis, insecticidal activity, action mechanism

谢晓俊, 羊桂英, 于保庭, 胡寅, 莫建初. 利用植物提取物绿色合成纳米银粒子及其对台湾乳白蚁的毒性[J]. 昆虫学报, 2022, 65(4): 480-489.

XIE Xiao-Jun, YANG Gui-Ying, YU Bao-Ting, HU Yin, MO Jian-Chu. Green synthesis of silver nanoparticles using plant extracts and their toxicity to Coptotermes formosanus (Blattodea: Rhinotermitidae)[J]. Acta Entomologica Sinica, 2022, 65(4): 480-489.

[1]	芦小鹏, 吴琼, 李毛龙, 李三兴, 马志卿, 张兴. 126种植物精油或精油主要成分对玉米黄呆蓟马的熏杀活性筛选[J]. , 2018, 61(8): 941-949.
[2]	张敏, 刘佳, 傅伟杰, 代光辉. 姜黄根提取物对苜蓿蚜成蚜有杀虫活性化合物的分离与鉴定[J]. , 2018, 61(6): 698-703.
[3]	赵静茹, 袁守丽, 高斌, 朱顺义. 抑制剂胱氨酸结多肽的抗菌和杀虫活性及进化[J]. , 2018, 61(10): 1212-1221.
[4]	Ali MEHRVAR. 荧光增白剂对伊朗核型多角体病毒分离株对棉铃虫幼虫的杀虫活性的增效作用（英文）[J]. , 2013, 56(6): 708-714.
[5]	黄继光, 赵欢欢, 苗辉, 徐汉虹. 羽裂蟹甲草对家蝇和白纹伊蚊的杀虫活性和活性成分(英文)[J]. , 2012, 55(12): 1355-1361.
[6]	李有志, 李冠华, 魏孝义, 刘仲华, 徐汉虹. 灰毛豆树皮中的杀虫成分及其杀虫活性（英文）[J]. , 2011, 54(12): 1368-1376.
[7]	邱思鑫，范晓静，洪鹏翔，关雄，胡方平. 苏云金芽胞杆菌营养期杀虫蛋白基因vip3A在枯草芽胞杆菌中的表达[J]. , 2010, 53(5): 508-516.
[8]	王学贵，沈丽淘，田永清，徐汉虹. 岩木瓜化学成分的分离、结构鉴定与杀虫活性[J]. , 2010, 53(5): 525-530.
[9]	李秀梅, 方继朝. 18种中草药提取物对褐飞虱和甜菜夜蛾的杀虫活性分析[J]. , 2010, 53(3): 298-306.
[10]	何玲，赵娟，师宝君，胡兆农，吴文君. 杠柳杀虫组分F3-28对东方粘虫和小地老虎幼虫中肠消化酶活性的影响[J]. , 2010, 53(11): 1248-1255.
[11]	黄素青, 徐汉虹, 童松, 张志祥. Interruptin B对小菜蛾和亚洲玉米螟幼虫的拒食活性及对斜纹夜蛾卵巢细胞的毒力[J]. , 2010, 53(10): 1104-1110.
[12]	张云, 彭映辉, 陈飞飞, 曾冬琴, 黄谊, 钟海雁. 椿叶花椒果实精油对两种蚊虫的生物活性及成分分析[J]. , 2009, 52(9): 1028-1033.
[13]	Abiodun A. DENLOYE, Oluwakemi K. AJELARA, Rasaq A. OLOWU, Adeolu O. ESHILOKUN, Winifred A. MAKANJUOLA. 土荆芥的石油醚提取物及精油对冈比亚按蚊的杀虫活性[J]. , 2009, 52(8): 923-928.
[14]	刘艳荷，方继朝，郭慧芳. 昆虫杆状病毒几丁质酶及其应用研究进展[J]. , 2008, 51(4): 430-436.
[15]	李洪波，夏玉先. 昆虫神经毒素BjαIT的表达及杀虫活性[J]. , 2008, 51(11): 1138-1143.

利用植物提取物绿色合成纳米银粒子及其对台湾乳白蚁的毒性

Green synthesis of silver nanoparticles using plant extracts and their toxicity to Coptotermes formosanus (Blattodea: Rhinotermitidae)

PDF (PC)

PDF (Mobile)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0