黑胸散白蚁工蚁与工蚁型补充生殖蚁体内抗氧化酶和解毒酶的活性及基因表达变化

doi:10.16380/j.kcxb.2022.10.003

昆虫学报 ›› 2022, Vol. 65 ›› Issue (10): 1266-1276.doi: 10.16380/j.kcxb.2022.10.003

黑胸散白蚁工蚁与工蚁型补充生殖蚁体内抗氧化酶和解毒酶的活性及基因表达变化

董亚楠^1,#, 牛童^1,#, 吴佳², 王超^1,*, 张贺^3,*

(1. 西北工业大学生态环境学院, 西安 710129; 2. 西安外事学院医学院, 西安 710077; 3. 郑州大学附属肿瘤医院病理科, 郑州 450008)

出版日期:2022-10-20 发布日期:2022-11-27

Changes in the activities and gene expression of antioxidant enzymes and detoxification enzymes in workers and ergatoid reproductives of Reticulitermes chinensis (Isoptera: Rhinotermitidae)

DONG Ya-Nan^1,#, NIU Tong^1,#, WU Jia², WANG Chao^1,*, ZHANG He^3,*

(1. School of Ecology and Environment, Northwestern Polytechnical University, Xi′an 710129, China; 2. College of Medicine, Xi′an International University, Xi′an 710077, China; 3. Department of Pathology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China)

Online:2022-10-20 Published:2022-11-27

摘要/Abstract

摘要： 【目的】社会性白蚁的工蚁可以转化成补充生殖蚁参与生殖，提高自身适合度。本研究旨在探究工蚁转变成补充生殖蚁后，与氧化应激抗衰老相关的抗氧化酶和解毒酶活性的变化，为揭示生殖蚁的繁殖和抗衰老机制提供参考。【方法】利用生化方法分别测定黑胸散白蚁Reticulitermes chinensis工蚁、工蚁型补充生殖蚁和原始生殖蚁体内的2种抗氧化酶过氧化氢酶(catalase, CAT)和超氧化物歧化酶(superoxide dismutase, SOD)与4种解毒酶酸性磷酸酶(acid phosphatase, ACP)、碱性磷酸酶(alkaline phosphatase, AKP)、羧酸酯酶(carboxylesterase, CarE)和细胞色素P450(cytochrome P450, CYP450)的酶活性；同时利用qRT-PCR检测这些酶对应的基因RsCAT, RsSOD, RsACP, RsCYP450和RsCarE的表达量。【结果】与雌性工蚁相比，黑胸散白蚁工蚁型补充生殖蚁体内的CAT, SOD, ACP, AKP和CarE活性显著上升，分别达到雌性工蚁的5.82, 1.41, 1.39, 2.27和2.70倍，CYP450活性在两品级间没有显著差异；RsCAT, RsSOD, RsACP, RsCarE和RsCYP450的表达量也显著增加。补充生殖蚁体内CAT和ACP活性显著高于原始生殖蚁的，而SOD和AKP活性在两品级间没有显著差异；雌雄补充生殖蚁RsCAT的相对表达量分别是原始生殖蚁的5.68和3.60倍，RsACP的相对表达量分别是原始生殖蚁的81.12和46.72倍。【结论】黑胸散白蚁工蚁由非生殖品级转化为生殖品级以后，体内抗氧化酶和解毒酶的酶活力和基因表达水平显著升高，从一定程度上揭示了生殖品级的抗衰老机制。

关键词: 黑胸散白蚁, 工蚁；工蚁型补充生殖蚁；原始生殖蚁；抗氧化酶；解毒酶；衰老

Abstract: 【Aim】 Social termite workers can differentiate into ergatoid reproductives to produce offsprings which is in favour of the fitness obtained. This study aims to explore the changes in the activities of antioxidant enzymes and detoxification enzymes related to oxidative stress and anti-aging when workers differentiate into ergatoid reproductives, so as to provide a reference for revealing the reproductive and anti-aging mechanism of reproductive castes of termites. 【Methods】 The activities of two antioxidant enzymes, catalase (CAT) and superoxide dismutase (SOD), and four detoxification enzymes, acid phosphatase (ACP), alkaline phosphatase (AKP), carboxylesterase (CarE) and cytochrome P450 (CYP450), in the workers, ergatoid reproductives and primary reproductives of Reticulitermes chinensis were measured by biochemical methods. Meanwhile, the expression levels of the corresponding genes of the above enzymes, RsCAT, RsSOD, RsACP, RsCYP450 and RsCarE, were assessed by qRT-PCR. 【Results】 The activities of CAT, SOD, ACP, AKP and CarE in ergtoid reproductives of R. chinensis were significantly higher than those in female workers, reaching 5.82, 1.41, 1.39, 2.27 and 2.70 times as high as those in female workers, respectively, but there was no significant difference in the CYP450 activity between the two castes. Also the expression levels of RsCAT, RsSOD, RsACP, RsCarE and RsCYP450 in ergtoid reproductives were significantly higher than those in workers. Compared with the primary reproductives, the ergatoid reproductives showed a significant increase in the activities of CAT and ACP. However, there was no significant difference in the activities of SOD and AKP between the two castes. The relative expression levels of RsCAT in female and male ergatoid reproductives were 5.68 and 3.60 times, respectively, and those of RsACP in female and male ergatoid reproductives were 81.12 and 46.72 times, respectively, as high as those in primary reproductives. 【Conclusion】 The activities and gene expression levels of antioxidant enzymes and detoxification enzymes significantly increase after the termite workers of R. chinensis develop from nonreproductive caste to reproductive caste, revealing the anti-aging mechanism of reproductives to a certain extent.

Key words: Reticulitermes chinensis, workers, ergatoid reproductives, primary reproductive, antioxidant enzyme, detoxification enzyme, aging

董亚楠, 牛童, 吴佳, 王超, 张贺. 黑胸散白蚁工蚁与工蚁型补充生殖蚁体内抗氧化酶和解毒酶的活性及基因表达变化[J]. 昆虫学报, 2022, 65(10): 1266-1276.

DONG Ya-Nan, NIU Tong, WU Jia, WANG Chao, ZHANG He. Changes in the activities and gene expression of antioxidant enzymes and detoxification enzymes in workers and ergatoid reproductives of Reticulitermes chinensis (Isoptera: Rhinotermitidae)[J]. Acta Entomologica Sinica, 2022, 65(10): 1266-1276.

黑胸散白蚁工蚁与工蚁型补充生殖蚁体内抗氧化酶和解毒酶的活性及基因表达变化

Changes in the activities and gene expression of antioxidant enzymes and detoxification enzymes in workers and ergatoid reproductives of Reticulitermes chinensis (Isoptera: Rhinotermitidae)

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