意大利蝗卵发育过程保幼激素和蜕皮激素含量的变化及其代谢相关基因的表达谱

doi:10.16380/j.kcxb.2023.02.001

昆虫学报 ›› 2023, Vol. 66 ›› Issue (2): 121-129.doi: 10.16380/j.kcxb.2023.02.001

• 研究论文 • 下一篇

意大利蝗卵发育过程保幼激素和蜕皮激素含量的变化及其代谢相关基因的表达谱

赵娜1, 叶小芳^1,*, 程文玉¹, 宋余¹, 蒋思涵¹, 王晗¹, 丁国婵², 季荣¹

(1. 新疆师范大学生命科学学院, 中亚区域跨境有害生物联合控制国际研究中心, 新疆特殊环境物种保护与调控生物学实验室, 新疆特殊环境物种多样性应用与调控重点实验室, 乌鲁木齐 830054; 2. 新疆玛纳斯县蝗虫鼠害预测预报防治站, 玛纳斯 832200)

出版日期:2023-02-20 发布日期:2023-04-07

Variation in the contents of juvenile hormone and ecdysone and expression profiling of their metabolism-related genes during the development of Calliptamus italicus (Orthoptera: Catantopidae) eggs

ZHAO Na¹, YE Xiao-Fang^1,*, CHENG Wen-Yu¹, SONG Yu¹, JIANG Si-Han¹, WANG Han¹, DING Guo-Chan², JI Rong¹

(1. Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, International Center for the Collaborative Management of Cross-border Pest in Central Asia, School of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; 2. Manasi Station of Forecast and Control on Locusts and Rodents, Manasi 832200, China)

Online:2023-02-20 Published:2023-04-07

摘要/Abstract

摘要： 【目的】鉴于保幼激素(juvenile hormone, JH)与蜕皮激素(ecdysone, Ecd)在昆虫生长发育中的重要作用，本研究旨在明确这两种激素及其代谢相关基因对意大利蝗Calliptamus italicus卵发育过程的调控机制。【方法】采用ELISA技术检测意大利蝗卵发育过程中JH与Ecd含量的变化；采用qRTPCR技术检测JH与Ecd代谢通路的重要基因(JHE, JHEH, DIB和EcR)在意大利蝗卵发育过程的表达模式。【结果】意大利蝗卵滞育阶段(阶段Ⅳ-Ⅵ)的JH含量显著高于早期发育阶段(阶段Ⅰ-Ⅲ)的，滞育后发育阶段(阶段Ⅶ-Ⅸ) JH含量显著下降；Ecd含量于滞育早期(阶段Ⅳ)显著上升，而后显著下降，滞育后发育阶段再次上升。JHE的表达量在意大利蝗卵早期发育阶段和滞育后发育阶段均呈先升后降的趋势，滞育阶段JHE的表达量较低；JHEH表达量在意大利蝗卵早期发育阶段也先升后降，滞育阶段变化不显著，滞育后发育阶段显著升高；DIB表达量在意大利蝗卵滞育阶段高于早期发育阶段的，在滞育后发育阶段下降；EcR表达量在意大利蝗卵早期发育阶段及滞育阶段均无显著变化，滞育后发育阶段逐渐升高。【结论】意大利蝗卵的发育过程受JH与Ecd共同调控，且JH为调控意大利蝗卵滞育进入的重要激素，Ecd为意大利蝗卵滞育解除的重要激素；意大利蝗卵的JH分解代谢通路在滞育解除前主要由JHE调控，滞育解除后主要由JHEH调控；DIB与EcR通过调节Ecd含量进而影响意大利蝗卵发育。这些结果为深入揭示蝗卵滞育机制奠定了基础。

关键词: 意大利蝗, 保幼激素, 蜕皮激素, JHE, JHEH；DIB, EcR

Abstract: 【Aim】Juvenile hormone (JH) and ecdysone (Ecd) play important roles in the growth and development of insects. The aim of this study is to clarify the regulatory mechanism of these two hormones and their metabolism-related genes on the development of Calliptamus italicus eggs.【Methods】The changes in contents of JH and Ecd during the development of C. italicus eggs were detected by ELISA assay, and the expression patterns of important genes (JHE, JHEH, DIB and EcR) in the metabolic pathways of JH and Ecd during the development of C. italicus eggs were detected by qRT-PCR.【Results】The JH content in C. italicus eggs was significantly higher at the diapause stage (stages Ⅳ-Ⅵ) than at the early developmental stage (stages Ⅰ-Ⅲ), and decreased significantly at the post-diapause developmental stage (stages Ⅶ-Ⅸ). The Ecd content in C. italicus eggs increased significantly at the early diapause stage (stage Ⅳ), then decreased significantly, and increased again at the post-diapause developmental stage. The expression level of JHE in C. italicus eggs increased firstly and then decreased at the early developmental stage and the post-diapause developmental stage, and was low at the diapause stage. The expression level of JHEH in C. italicus eggs also increased first and then decreased at the early developmental stage, and didn’t change significantly at the diapause stage, but increased significantly at the post-diapause developmental stage. The expression level of DIB in C. italicus eggs was higher at the diapause stage than at the early developmental stage and decreased at the post-diapause developmental stage. The expression level of EcR in C. italicus eggs did not change significantly at the early developmental stage and diapause stage, but increased gradually at the post-diapause developmental stage. 【Conclusion】The development of C. italicus eggs is co-regulated by JH and Ecd. JH is the important hormone to regulate diapause entry, while Ecd is the important hormone for diapause termination of C. italicus eggs. JH catabolic pathway of C. italicus eggs is mainly regulated by JHE before diapause termination and by JHEH after diapause termination. DIB and EcR can affect the development of C. italicus eggs by regulating Ecd content. These results lay a foundation for further revealing the diapause mechanism of C. italicus eggs.

Key words: Calliptamus italicus, juvenile hormone, ecdysone, JHE, JHEH, DIB, EcR

赵娜, 叶小芳, 程文玉, 宋余, 蒋思涵, 王晗, 丁国婵, 季荣. 意大利蝗卵发育过程保幼激素和蜕皮激素含量的变化及其代谢相关基因的表达谱[J]. 昆虫学报, 2023, 66(2): 121-129.

ZHAO Na, YE Xiao-Fang, CHENG Wen-Yu, SONG Yu, JIANG Si-Han, WANG Han, DING Guo-Chan, JI Rong. Variation in the contents of juvenile hormone and ecdysone and expression profiling of their metabolism-related genes during the development of Calliptamus italicus (Orthoptera: Catantopidae) eggs[J]. Acta Entomologica Sinica, 2023, 66(2): 121-129.

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意大利蝗卵发育过程保幼激素和蜕皮激素含量的变化及其代谢相关基因的表达谱

Variation in the contents of juvenile hormone and ecdysone and expression profiling of their metabolism-related genes during the development of Calliptamus italicus (Orthoptera: Catantopidae) eggs

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