关键词: 中华按蚊, 抗性, 共生微生物, 多样性, 生物信息学

Abstract: 【Aim】 To investigate the relationship between the symbiotic microbial communities in Anopheles sinensis and the insecticide resistance of An. sinensis in different regions of the wild environment. 【Methods】 Wild female adults of An. sinensis were collected from Chongqing, Yunnan, and Anhui in China. WHO standard in vitro bioassay and 0.05% deltamethrin impregnated paper were used to conduct insecticide susceptibility tests on the female adults of An. sinensis, classifying them as insecticide-sensitive (FS) or insecticide-resistant (FR) to perform high-throughput sequencing of the whole genomes through Illumina Hiseq 2000 platform. The sequences of 16S rRNA and 18S rRNA genes were aligned to identify symbiotic microbiota in the FS and FR An. sinensis. Alpha diversity analysis, beta diversity analysis, clustering analysis, principal coordinates analysis (PCoA) and analysis of symbiotic microbial community differences were conducted. 【Results】 A total of 3 284 species of 52 genera of 14 phyla of symbiotic microbiota were identified from the female adults of the FS and FR wild An. sinensis from Chongqing, Yunnan and Anhui. The diversity difference and community richness of the FR wild An. sinensis female adults from Anhui were the highest, and those of the FS wild An. sinensis female adults from Chongqing were the lowest. The symbiotic microbial communities in the female adults of wild An. sinensis were first clustered by region, and the diversity of the symbiotic microbial communities in the female adults of An. sinensis from Chongqing and Yunnan were more similar. A total of 10 strains including two strains of Cyanobacteria, three strains of Halobacteria, one strain of Haliangium, three strains of Ruminococcaceae and one strain of Streptococcus of bacteria in the symbiotic microbiota in the FR wild An. sinensis female adults were more abundant.【Conclusion】 The symbiotic microbial communities of the wild An. sinensis exhibit regional and resistance differences. The identified symbiotic microbiota play a positive role in the insecticide resistance of An. sinensis. The results of this study provide insights into understanding the insecticide resistance of An. sinensis.

Key words: Anopheles sinensis, resistance, symbiotic microbiota, diversity, bioinformatics