基于线粒体Cytb的新疆黑条小车蝗种群遗传多样性及谱系地理学研究

doi:10.16380/j.kcxb.2025.01.010

昆虫学报 ›› 2025, Vol. 68 ›› Issue (1): 98-109.doi: 10.16380/j.kcxb.2025.01.010

基于线粒体Cytb的新疆黑条小车蝗种群遗传多样性及谱系地理学研究

马钰洁^1,2,3, 张晓清^1,2,3, 王一鸣^1,2,3, 钟圆佳^1,2,3, 季荣^1,2,3, 袁亮^1,2,3,*

(1. 新疆师范大学生命科学学院, 中亚区域跨境有害生物联合控制国际联合研究中心, 乌鲁木齐 830054; 2. 新疆师范大学生命科学学院, 新疆特殊环境物种保护与调控生物学实验室, 乌鲁木齐 830054; 3. 新疆师范大学生命科学学院, 新疆特殊环境物种多样性应用与调控重点实验室, 乌鲁木齐 830054)

出版日期:2025-01-20 发布日期:2025-02-25

Genetic diversity and phylogeography of Oedaleus decorus decorus (Orthoptera: Oedipodidae) populations in Xinjiang, western China based on the mitochondrial Cytb

MA Yu-Jie^1,2,3, ZHANG Xiao-Qing^1,2,3, WANG Yi-Ming^1,2,3, ZHONG Yuan-Jia1^,2,3, JI Rong^1,2,3, YUAN Liang^1,2,3,*

(1. International Center for the Collaborative Management of Crossborder Pests in Central Asia, School of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; 2. Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, School of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; 3. Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, School of Life Sciences, Xinjiang Normal University, Urumqi 830054, China)

Online:2025-01-20 Published:2025-02-25

摘要/Abstract

摘要： 【目的】本研究旨在揭示中国西部新疆地区黑条小车蝗Oedaleus decorus decorus不同地理种群的遗传多样性及谱系关系，为新疆迁飞蝗虫的历史迁飞规律提供分子遗传学证据。【方法】PCR扩增新疆28个黑条小车蝗地理种群线粒体Cytb上740 bp的核酸序列，基于Cytb片段序列利用DnaSP 5.10和Arlequin 3.1软件对黑条小车蝗地理种群的遗传多样性、遗传分化格局和基因流等进行分析。【结果】从新疆28个黑条小车蝗地理种群的338条Cytb片段中共检出27个单倍型，群体单倍型多样性指数(Hd)为0.8185，核苷酸多样性(π)为0.00611，暗示黑条小车蝗种群遗传多样性水平较高；基于单倍型构建的分子系统发育树及median-joining网络关系分析表明，新疆地区黑条小车蝗地理种群被明显划分为2个地理遗传谱系：天山北麓谱系(谱系Ⅰ，包括温泉、沙湾、昌吉、乌鲁木齐和哈密种群及博乐种群的1头个体)和中哈边境谱系(谱系Ⅱ，包括伊犁、塔城和阿勒泰种群及博乐种群的11头个体)；AMOVA分析显示新疆黑条小车蝗不同地理种群间的遗传变异较高(71.38%)，而不同地理种群内的遗传变异水平较低(28.62%)； 28个地理种群间的遗传分化指数(Fst)为-0.097~0.987， 2个地理遗传谱系之间遗传分化指数(Fst)为0.805；核苷酸错配分布呈现多峰，Tajima’s D值为0.193，Fu’s Fs为-2.863，推测新疆地区黑条小车蝗未发生历史种群扩张。【结论】新疆地区黑条小车蝗具有丰富的遗传多样性和明显的系统地理分布格局； 2个地理谱系间基因交流较弱，存在较大遗传分化，但2个地理谱系内各种群存在较强基因流，推测是高山、沙漠等地理隔离所致。该研究结果为重构新疆草原蝗虫历史迁飞路径及阐明当前分布格局提供分子遗传学依据。

关键词: 黑条小车蝗, Cytb, 遗传多样性, 谱系地理学, 基因流

Abstract: 【Aim】 The aim of this study is to reveal the genetic diversity and genealogical relationships of different geographic populations of Oedaleus decorus decorus in Xinjiang, western China, and to provide molecular genetic evidence for the historical migratory patterns of migratory locusts in Xinjiang.【Methods】 PCR was used to amplify the 740 bp of nucleic acid sequence on the mitochondrial Cytb of 28 geographic populations of O. decorus decorus in Xinjiang. The genetic diversity, patterns of genetic differentiation and gene flow in geographic populations of O. decorus decorus were analyzed based on Cytb fragment sequences using DnaSP 5.10 and Arlequin 3.1 software. 【Results】 A total of 27 haplotypes were detected in 338 Cytb fragments from 28 geographic populations of O. decorus decorus in Xinjiang, and the population haplotype diversity index (Hd) was 0.8185 and the nucleotide diversity (π) was 0.00611, implying a high level of population genetic diversity of O. decorus decorus populations. Molecular phylogenetic trees constructed based on haplotypes and median-joining network relationship analyses showed that the geographic populations of O. decorus decorus in Xinjiang were clearly divided into two geographic genetic lineages: The northern Tianshan foothills lineage (lineage I, including Wenquan, Shawan, Changji, Urumqi and Hami populations, and 1 individual from the Bole population) and the Sino-Harbin border lineage (lineage II, including Ili, Tacheng and Altay populations, and 11 individuals from the Bole population). AMOVA analysis showed high genetic variation (71.38%) among different geographic populations of O. decorus decorus in Xinjiang, while the level of genetic variation within different geographic populations was low (28.62%). The genetic differentiation index (Fst) among the 28 geographic populations ranged from -0.097 to 0.987, and that between the two geographic genetic lineages was 0.805. The distribution of nucleotide mismatches showed multiple peaks, with the Tajima’s D value of 0.193 and Fu’s Fs value of -2.863, hypothesizing that no historical population expansion of O. decorus decorus had occurred in Xinjiang. 【Conclusion】 O. decorus decorus had rich genetic diversity and distinct phylogeographical patterns in Xinjiang. Gene exchange between the two geographic lineages was weak and there was large genetic differentiation, but there was strong gene flow for various populations within the two geographic lineages, presumably due to geographic isolation such as high mountains and deserts. The results of this study provide a molecular genetic basis for reconstructing the historical migratory paths of locusts in Xinjiang grassland and elucidating the current distribution pattern.

Key words: Oedaleus decorus decorus, Cytb, genetic diversity, phylogeography, gene flow

马钰洁, 张晓清, 王一鸣, 钟圆佳, 季荣, 袁亮. 基于线粒体Cytb的新疆黑条小车蝗种群遗传多样性及谱系地理学研究[J]. 昆虫学报, 2025, 68(1): 98-109.

MA Yu-Jie, ZHANG Xiao-Qing, WANG Yi-Ming, ZHONG Yuan-Jia, JI Rong, YUAN Liang. Genetic diversity and phylogeography of Oedaleus decorus decorus (Orthoptera: Oedipodidae) populations in Xinjiang, western China based on the mitochondrial Cytb[J]. Acta Entomologica Sinica, 2025, 68(1): 98-109.

[1]	冯新颖, 王璇, 李冰艳, 曾彪, 李虎, 刘志琦, 宋凡. 基于线粒体基因组的云南省橘小实蝇种群遗传结构分析[J]. 昆虫学报, 2025, 68(1): 110-122.
[2]	王琦琦, 孙艳, 唐光辉. 基于九个微卫星标记的核桃举肢蛾地理种群的遗传多样性分析 [J]. 昆虫学报, 2024, 67(1): 90-101.
[3]	袁彬乔, 赵向杰, 侯珲, 张金勇, 黄天祥, 涂洪涛. 中国橘小实蝇不同地理种群遗传多样性分析[J]. 昆虫学报, 2023, 66(2): 235-244.
[4]	王佰秋, 沈初泽, 武雪, 谢慧聪, 宗靖淞, 余哲媛, 白芸, 李恺, 何祝清. 基于微卫星分子标记的浙江省不同滞育型迷卡斗蟋的遗传分化分析[J]. 昆虫学报, 2022, 65(6): 782-790.
[5]	唐相友, 宋华莉, 石鹏, 张小燕, 唐紫寒, 王文峰, 扎罗, 陈新兰, 周泽扬, 许金山. 全基因组重测序揭示青藏高原东缘及东南缘东方蜜蜂遗传多样性与适应性进化[J]. 昆虫学报, 2022, 65(5): 638-647.
[6]	罗林丽, 孟泽洪, 李帅, 赵兴丽, 周罗娜, 贺圣凌, 魏茹蕙, 张欣, 周玉锋. 中国南方茶棍蓟马地理种群遗传分化分析[J]. 昆虫学报, 2022, 65(4): 500-511.
[7]	陈蓉, 牛力康, 端木浩楠, 王宗庆, 车艳丽. 线粒体基因和核基因揭示自然屏障和第四纪更新世气候振荡影响双斑乙蠊谱系地理格局(英文)[J]. 昆虫学报, 2022, 65(2): 235-245.
[8]	钟裕俊, 杜素洁, 潘立婷, 王玉生, 王福莲, 刘万学. 基于mtCOI基因序列的中国葱斑潜蝇不同地理种群遗传多样性分析[J]. 昆虫学报, 2020, 63(6): 769-778.
[9]	李奎, 周传江, 梁爱萍. 基于线粒体16S rRNA基因的舟山群岛碧蛾蜡蝉种群遗传多样性分析[J]. 昆虫学报, 2020, 63(4): 502-511.
[10]	王梦琦, 张利娟, 张宏瑞, 孙跃先. 中国北方黑唇苜蓿盲蝽种群遗传多样性和遗传分化分析[J]. 昆虫学报, 2020, 63(12): 1516-1524.
[11]	徐浩, 陈孝梅, 蔺哲广, 吉挺. 湖南龙山不同海拔中华蜜蜂种群遗传多样性的微卫星DNA分析[J]. 昆虫学报, 2020, 63(10): 1260-1267.
[12]	姜姗, 王兴亚, 王小奇. 基于微卫星标记的中国东北地区灰飞虱遗传变异及种群遗传结构分析[J]. 昆虫学报, 2020, 63(1): 73-84.
[13]	崔中翌, 周琼, 刘一鹏, 司品法, 汪洋. 基于mtDNA COI基因序列的中国柑橘果实蝇的分子鉴定及柑橘大实蝇种群的遗传多样性分析[J]. 昆虫学报, 2020, 63(1): 85-96.
[14]	洪波, 张锋, 陈志杰, 罗坤, 赵惠燕. 基于微卫星标记的中国枣食芽象甲地理种群遗传多样性分析[J]. 昆虫学报, 2019, 62(3): 381-390.
[15]	王克勤, 刘兴龙, 邵天玉. 中国东北地区亚洲玉米螟遗传多样性及寄主专化性分析[J]. , 2018, 61(9): 1054-1066.

基于线粒体Cytb的新疆黑条小车蝗种群遗传多样性及谱系地理学研究

Genetic diversity and phylogeography of Oedaleus decorus decorus (Orthoptera: Oedipodidae) populations in Xinjiang, western China based on the mitochondrial Cytb

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0