桃蚜热激蛋白Hsp70基因在高、低温胁迫下的表达与功能分析

doi:10.16380/j.kcxb.2025.07.001

昆虫学报 ›› 2025, Vol. 68 ›› Issue (7): 877-886.doi: 10.16380/j.kcxb.2025.07.001

• 研究论文 • 下一篇

桃蚜热激蛋白Hsp70基因在高、低温胁迫下的表达与功能分析

刘嘉睿¹, 孟建玉², 董祥立³, 李治模³, 杨昌利¹, 张长禹^1,*

(1. 贵州大学昆虫研究所, 贵州省山地农业病虫害重点实验室, 贵阳 550025; 2. 贵州省烟草科学研究院, 贵阳 550081; 3. 贵州省烟草公司遵义市公司凤冈县分公司, 遵义 564200)

出版日期:2025-07-20 发布日期:2025-08-25

Expression and functional analysis of the heat shock protein Hsp70 genes in Myzus persicae (Hemiptera: Aphididae) under high and low temperature stress

LIU Jia-Rui¹, MENG Jian-Yu², DONG Xiang-Li³, LI Zhi-Mo³, YANG Chang-Li¹, ZHANG Chang-Yu^1,*

(1. Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China; 2. Guizhou Tobacco Science Research Institute, Guiyang 550081, China; 3. Guizhou Province Tobacco Company Zunyi City Company Fenggang County Branch, Zunyi 564200, China)

Online:2025-07-20 Published:2025-08-25

摘要/Abstract

摘要： 【目的】探究桃蚜Myzus persicae 热激蛋白Hsp70基因在桃蚜响应高、低温胁迫中的作用。【方法】利用RT-qPCR检测桃蚜8个MpHsp70基因(MpHsp70-1, MpHsp70-2, MpHsp70A1, MpHsp70B2, MpHsp68a, MpHsp68b, MpHsc70-4和MpHsp70)在桃蚜无翅成蚜不同组织(头、中肠、胚胎和表皮)及分别在高温(36 ℃)和低温(4 ℃)胁迫下不同时长(0, 30, 60, 90, 120和150 min)无翅成蚜中的相对表达量；利用RNAi沉默两个关键MpHsp70基因(MpHsp70A1和MpHsp68a)，观测无翅成蚜在高温(36 ℃)处理120 min和低温(4 ℃)处理30 min后无翅成蚜存活率和产若蚜量。【结果】 8个MpHsp70基因在桃蚜无翅成蚜不同组织中均表达，其中MpHsp70, MpHsp70A1, MpHsp70B2, MpHsc70-4和MpHsp68b在表皮中的表达量显著高于在其他组织中的，MpHsp70-1, MpHsp70-2和MpHsp68a在胚胎中的表达量显著高于在其他组织中的；高、低温胁迫对桃蚜无翅成蚜MpHsp70基因表达具有明显的诱导作用， 4 ℃胁迫下，MpHsp70-1, MpHsp70A1, MpHsp70-2, MpHsp68a和MpHsp68b的表达量呈现出现上升后下降趋势，在4 ℃胁迫30 min时达到最高，显著高于对照的； 36 ℃胁迫下， MpHsp70-1, MpHsp70A1, MpHsp70-2, MpHsc70-4, MpHsp68a和MpHsp68b的表达量呈现先上升后下降的趋势， MpHsp70-1的表达量在36 ℃胁迫下60 min时达到最高，其他基因的表达量均在120 min时达到最高。与对照组相比，沉默MpHsp68a的表达后，高、低温胁迫下桃蚜成蚜的存活率和产若蚜量均显著下降；沉默MpHsp70A1的表达后，高温胁迫下桃蚜的存活率和产若蚜量均极显著下降。【结论】桃蚜MpHsp70基因可以响应高、低温胁迫，在桃蚜抵抗温度胁迫的分子机制中发挥着重要作用。

关键词: 桃蚜, Hsp70, 环境胁迫, 基因表达, 基因功能

Abstract: 【Aim】To explore the role of heat shock protein Hsp70 genes of Myzus persicae in response to high and low temperature stress. 【Methods】 RT-qPCR was used to detect the relative expression levels of eight MpHsp70 genes (MpHsp70-1, MpHsp70-2, MpHsp70A1, MpHsp70B2, MpHsp68a, MpHsp68b, MpHsc70-4 and MpHsp70) in different wingless adult tissues (head, midgut, embryo and cuticle) and wingless adults under high temperature (36 ℃) and low temperature (4 ℃) stress for different duration (0, 30, 60, 90, 120 and 150 min), respectively. RNAi was used to silence two key MpHsp70 genes (MpHsp70A1 and MpHsp68a), and the survival rate and number of nymphs produced of wingless adults were observed and calculated at 120 min under high temperature treatment (36 ℃) and 30 min under low temperature treatment (4 ℃).【Results】 The eight MpHsp70 genes were expressed in different wingless adult tissues of M. persicae, and the expression levels of MpHsp70, MpHsp70A1, MpHsp70B2, MpHsc70-4 and MpHsp68b in the cuticile were significantly higher than those in the other tissues. The expression levels of MpHsp70-1, MpHsp70-2 and MpHsp68a in the embryo of M. persicae were significantly higher than those in other tissues. High and low temperature stress had significant induction effect on the expression of MpHsp70 genes in wingless adult of M. persicae. The expression levels of MpHsp70-1, MpHsp70A1, MpHsp70-2, MpHsp68a and MpHsp68b all increased and then decreased under 4 ℃ stress, and reached the highest at 30 min under 4 ℃ stress, which were significantly higher than those of the control. Under 36 ℃ stress, the expressions levels of MpHsp70-1, MpHsp70A1, MpHsp70-2, MpHsc70-4, MpHsp68a and MpHsp68b increased first and then decreased. The expression level of MpHsp70-1 reached the highest at 60 min after 36 ℃ stress, and those of the other genes reached the highest at 120 min after 36 ℃ stress. After the silence of MpHsp68a, the survival rate and number of nymphs produced of M. persicae under high and low temperature stress were significantly decreased and those after the silence of MpHsp70A1 under high temperature stress were extremely significantly decreased as compared with those of the control group. 【Conclusion】MpHsp70 genes of M. persicae can respond to high and low temperature stress, and play an important role in the molecular mechanism of resistance to temperature stress of M. persicae.
Key words:

Key words: Myzus persicae, Hsp70, environmental stress, gene expression, gene function

刘嘉睿, 孟建玉, 董祥立, 李治模, 杨昌利, 张长禹. 桃蚜热激蛋白Hsp70基因在高、低温胁迫下的表达与功能分析[J]. 昆虫学报, 2025, 68(7): 877-886.

LIU Jia-Rui, MENG Jian-Yu, DONG Xiang-Li, LI Zhi-Mo, YANG Chang-Li, ZHANG Chang-Yu. Expression and functional analysis of the heat shock protein Hsp70 genes in Myzus persicae (Hemiptera: Aphididae) under high and low temperature stress[J]. Acta Entomologica Sinica, 2025, 68(7): 877-886.

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桃蚜热激蛋白Hsp70基因在高、低温胁迫下的表达与功能分析

Expression and functional analysis of the heat shock protein Hsp70 genes in Myzus persicae (Hemiptera: Aphididae) under high and low temperature stress

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