关键词: 星豹蛛, 谷胱甘肽S-转移酶(GST), 溴氰菊酯, 基因表达

Abstract: 【Aim】To clarify the spatiotemporal expression patterns of two genes of glutathione S-transferases (GSTs) in Pardosa astrigera and to investigate whether the two genes can respond to deltamethrin stress.【Methods】 Based on the transcriptome database of P. astrigera, the full-length cDNA sequences of the GST genes PaGSTd1 and PaGSTd2 of P. astrigera were cloned by PCR and bioinformatically analyzed. RT-qPCR was used to detect the expression levels of PaGSTd1 and PaGSTd2 in different developmental stages (2nd-6th instar nymphs and adult), female and male adult tissues (cephalothorax, abdomen and leg)， and in male adult of P. astrigera stressed by deltamethrin at different concentrations ［LC₁₀(5.151 mg/L), LC₃₀ (8.619 mg/L) and LC₅₀ (12.311 mg/L)］ for different time (6, 12, 18, 24 and 48 h)through residual film method.【Results】 The full-length cDNA sequence of PaGSTd1 (GenBank accession number: OR096398) of P. astrigera is 708 bp in length with an open reading frame of 645 bp in length, encoding 214 amino acids. The full-length cDNA sequence of PaGSTd2 (GenBank accession number: OR096399) of P. astrigerais 793 bp in length with an open reading frame of 645 bp in length, encoding 214 amino acids. The detection results of RT-qPCR showed that PaGSTd1 and PaGSTd2 were expressed in different developmental stages and adult tissues of P. astrigera, and the expression levels of PaGSTd1 and PaGSTd2 were the lowest in the 5th instar nymph. The expression level of PaGSTd1 was the highest in the 4th instar nymph of P. astrigera,and that of PaGSTd2 was the highest in adult of P. astrigera. The expression levels of PaGSTd1 and PaGSTd2 were the highest in the leg, and the expression levels in other tissues except for that in the abdomen were significantly higher in male adults than in female adults of P. astrigera. PaGSTd1 and PaGSTd2 in male adult of P. astrigera were induced at 24 h, and at 6 and 18 h, respectively, after stress by LC₁₀ of deltamethrin. PaGSTd1 and PaGSTd2 in male adult of P. astrigera were induced at 18, 24 and 48 h, and at 18 h, respectively, after stress by LC₃₀ of deltamethrin. Both PaGSTd1 and PaGSTd2 were induced in male adult of P. astrigera at 24 h after stress by LC₅₀ of deltamethrin.【Conclusion】 In this study, two GST genes PaGSTd1 and PaGSTd2 were cloned from P. astrigera. Both PaGSTd1 and PaGSTd2 were most highly expressed in the leg of P. astrigera, suggesting that these two GST genes in the leg of P. astrigera play an important role in the detoxification of exogenous substances. These two GST genes could be induced to express by deltamethrin, suggesting that these two GST genes PaGSTd1 and PaGSTd2 may be involved in the stress response of P. astrigera to deltamethrin, providing clues for subsequent functional studies of GSTs.

Key words: Pardosa astrigera, glutathione S-transferase (GST), deltamethrin, gene expression