关键词: 中华硬蜱, 二棘血蜱, 交叉免疫反应

Abstract: A comparative study was conducted for the first time to test the cross immune reaction between rabbits sensitized by bites of lxodes sinensis and Haernaphysalis bispinosa. When H. Bispinosa fed on the rabbits which had been attacked for three times previously by I. Sinensis,its body weight increased by 143.12±32.67 mg. But when it fed on the controlled rabbits notattacked previously by ticks the body weight increased by 181.30± 44.35 rog, which showed significant difference in comparison with the former case. The homogenates of I. Sinensis and H. bistnnosa snowed 24 and 22 proteins and protein subunits respectively in the SDS-PAGE patterns. The main bands of I. Sinensis were 6 and their molecular weights were 142, 105, 94,66/65, 64 and 58 KD. The main bands of H. bispinosa were 5 and their molecular weights were 215, 114, 105, 66/65 and 52 KD. The number of protein bands of H. bisplnosa and I.Sinensis were recognizable by the sera of rabbits attacked by H. bispinosa and by I. Sinensis,and they both contained an immunoblot, the common antigen band identified which is a 105KD protein band. It is concluded that the immune response of the rabbits attacked by these two ticks stimulates the production of antibodies which show cross immune reactions and the 105 KD protein may serve as the antigen common in both ticks.

Key words: lxodes sinenxis——Haernaphysalis bispinosa——trans immune reaction