›› 1997, Vol. 40 ›› Issue (2): 113-121.

• 研究论文 •    下一篇

棉铃虫对菊酯类杀虫剂抗药性的神经电生理研究

张友军 韩熹莱 张文吉 罗林儿 周培爱   

  • 出版日期:1997-05-20 发布日期:1997-05-20

AN ELECTROPHYSIOLGICAL STUDY ON RESISTANCE TO PYRETHROID INSECTICIDES IN HELICOVERPAARMIGERA (HUBNER)

hang You jun Han Xilai Zhang Wenji Luo Liner Zhou Peiai   

  • Online:1997-05-20 Published:1997-05-20

摘要: 本文用神经电生理方法研究了氰戊菊酯、氯菊酯对棉铃虫Helicoverpa armigera(Hubner)相对敏感(HD-S)种群和抗性(HJ-R)种群的神经毒理作用。10-5mol/L的氰戊菊酯、10-5mol/L的氯菊酯诱发腹神经索自发发放频率的增加和随后的神经传导阻断,10--5mol/l的氯菊酯抑制HD-S种群的神经兴奋,直接阻断神经传导。以兴奋时间、神经传导阻断时间、对药剂作用反应时间的个体分布频率3个参数比较两种群对杀虫剂的反应,均发现HJ-R种群比相对HD-S种群表现了2~3倍的神经不敏感机制,并且发现这种神经不敏感机制对毒理I型和Ⅱ型拟除虫菊酯同样有作用。

关键词: 棉铃虫, 电生理, 自发发放, 氰戊菊酯, 氯菊酯

Abstract: The effects of pyrethroids fenvalerate and permethrin were studies upon isolated abdominal nerve cords from the susceptible (HD-S) and resistant (HJ-R) strains of Helicoverpa armigera (Htibner). 10-5 mol/L fenvalerate and 10-5 mol/L permethrin induced increases in spontaneous firing frequency primarily and then led to cessation in both strains; 10-4 mol/L permethrin blocked the neural activity directly without producing repetitive discharges. On the basis of the time of excitation or cessation, there was a consistent pattern showing that the nervous system of HJ-R is 2- 3 times more resistant to pyrethroid actionshan that of HD-S. This experiment implied that there is a resistant mechanism of decreased nervous sensitity to pyrethroids in the field strain of Heliothis armigera in China and the mechanism conferred resistance to type I or II pyrethroids.

Key words: Helicoverpa armigera (Hubner), electrophysiology, spontaneous discharge, fenvalerate, permethrin