›› 1998, Vol. 41 ›› Issue (1): 21-26.doi:

• 研究论文 • 上一篇    下一篇

棉铃虫卵内蛋白酶性质研究

赵小凡 王金星 王德红   

  • 出版日期:1998-02-20 发布日期:1998-02-20

STUDIES ON THE PROPERTIES OF THE EGG PROTEINASES IN HELICOVERPA ARMIGERA

Zhao Xiaofan Wang Jinxing Wang Dehong   

  • Online:1998-02-20 Published:1998-02-20

摘要: 在棉铃虫Helicoverpa armigera卵母细胞内检测到蛋白酶活性,其作用Ph在酸性范围,酶活性受E-64、Pepstatin和iPr2P-F等多种抑制剂抑制。在Ph4.0时蛋白酶对牛血红蛋白有较高水解率。抗蓖麻蚕Philosamia cynthia ricini卵半胱氨酸蛋白酶血清和抗蓖麻蚕卵天冬氨酸蛋白酶血清可以识别棉铃虫卵内成分。实验结果表明;棉铃虫卵内可能存在半胱氨酸蛋白酶类、丝氨酸蛋白酶类和天冬氨酸蛋白酶类,并且与蓖麻蚕卵内蛋白酶有一定的相似性。

关键词: 棉铃虫, 卵内蛋白酶, 性质

Abstract: A proteolytic activity was detected in the developing oocytes of Helicoverpa armigera. The optimum Ph was in the acidic region. The activity could be inhibited by E-64, pepstatin and iPr2P-F. A highest hydrolysis rate was detected at Ph 4.0 when bovine hemoglobin was used as substrate. The antiserums to the cysteine proteinase and aspartic proteinase of Philosamia cynthia ricini could recognize the antigenes in the oocytes of H. armigera. The results suggested that there might be cysteine proteinase, serine proteinase and aspartic proteinase in the oocytes ofH. armigera, and these proteinases are similar to those in P. cynthia ricini.