›› 1998, Vol. 41 ›› Issue (3): 231-236.

• 研究论文 • 上一篇    下一篇

五带虻溶纤活性蛋白的纯化和性质

杨星勇 卢晓风 裴炎   

  • 出版日期:1998-08-20 发布日期:1998-08-20

PURIFICATION AND CHARACTERIZATION OF THE FIBRINOLYTIC COMPONENT OFTABANUS QUINQUECINCTUS RICARDO

Yang Xingyong Lu Xiaofeng Pei Yan   

  • Online:1998-08-20 Published:1998-08-20

摘要: 五带虻Tabanus qutnquectnctus Rlcardo腹部匀浆液经硫酸铵沉淀、Sephadex G-75凝胶层析、Fibrin-Sepharose 4B亲和层析和电泳制备等方法纯化后,获得在SDS—PAGE图谱上呈现单一区带的溶纤活性蛋白。该蛋白质既具有纤溶酶作用,又具有激活纤溶酶原的作用,其分子量为40kD,等电点为4.5,最适作用pH为9.0,最适作用温度为28℃,37℃处理2h活性完全丧失,Ca2+、Mn2+、Cu2+、Zn2+、Hg2+和PMSF能抑制其活性。

关键词: 五带虻, 溶纤活性蛋白

Abstract: The fibrinolytic component (FC) separated from the homogenate of abdomen of Tabanus quinquecinctus Ricardo was showed to possess both fibrinolytic enzyme and plasminogen activator activities. After ammonium sulphate precipitation,Sephadex G-75 gel filtration, Fibrin-Sepharose 4B affinity chromatography and elution from eleetrophoresis, it appeared as a single band corresponding to molecular weight(MW) of approximately 40kD on SDS-PAGE and its isoelectric point was about 4. 5 on IEF. Its optimal reaction pH was 9.0 and optimal reaction temperature was 28℃. The activity of FC was totally lost after incubation at 37℃ for two hours and could be inhibited byCa2+, Mn2+, Cu2+, Zn2+, Hg2+and PMSF.

Key words: Tabanus quinquecinctus Recardo, fibrinolytic component