›› 1999, Vol. 42 ›› Issue (4): 353-357.

• 研究论文 • 上一篇    下一篇

保幼激素类似物对德国小蠊卵黄发生及繁殖的影响

于长明, 刘泉, 李成文   

  • 出版日期:1999-11-20 发布日期:1999-11-20

EFFECTS OF PYRIPROXYFEN ON VITELLOGENESIS AND REPRODUCTION OF THE GERMAN COCKROACH, BLATTELLA GERMANICA

Yu Chang-ming,Liu Quan,Li Cheng-wen   

  • Online:1999-11-20 Published:1999-11-20

摘要: 应用ELISA、单向免疫扩散法,研究了保幼激素类似物(灭幼宝)对德国小蠊Blattella germanica卵黄发生的影响;同时,观察了灭幼宝对德国小蠊繁殖的影响。灭幼宝促进了德国小蠊脂肪体Vg的合成,使血淋巴Vg的浓度明显增高,出现时间早于对照组;且变化规律不同于对照组,呈双峰模式。灭幼宝处理组昆虫卵巢中Vt的含量在第5天、第7天明显高于对照组,而在第9天明显下降,仅为对照组的50%左右,而此时对照组卵巢的Vt含量则上升到处理组第7天水平。灭幼宝处理新羽化的雌虫,导致不育。

关键词: 灭幼宝, 卵黄原蛋白, 卵黄蛋白, 德国小蠊, 繁殖

Abstract: As a juvenile hormone analog (JHA), pyriproxyfen has showed a very high efficacy in cockroach control. The effects of JHAs on vitellogenesis and reproduction in many insects have been widely reported. In this study, the effect of pyriproxyfen on the vitellogenesis and reproduction of Blattella germanica has been investigated by employing immunological and biochemical methods. When the female adults were treated with pyriproxyfen, the synthesis of vitellogenin in fat body was promoted, and the biomodel of vitellogenin in haemolymph exhibited a very different pattern, i.e. 2 peaks of vitellogenin content. Vt began to accumulate earlier and maintained at a very high level for several days, then decreased quickly afterward in the ovary of treated female. At the 9th day the Vt content lowered to half of the peak level in the normal adult of the same day-age. When the newly emerged female adults were treated with this JHA, they became sterilized.

Key words: pyriproxyfen, vitellogenin, vitellin, Blattella germanica, reproduction