阿维菌素对小菜蛾的抗性选育及其对解毒酶活性的影响

›› 2000, Vol. 43 ›› Issue (-1): 38-43.

阿维菌素对小菜蛾的抗性选育及其对解毒酶活性的影响

李腾武，高希武，郑炳宗，许向丽

出版日期:2000-12-20 发布日期:2000-12-20

Author for correspondenceStudy on resistance selection by avermectins and its effect onactivities of detoxification enzymes inPlutella xylostella(L.)

LI Teng-wu, GAO Xi-wu, ZHENG Bing-zong, XU Xiang-li

Online:2000-12-20 Published:2000-12-20
Contact: GAO Xi-wu

摘要/Abstract

摘要： 用阿维菌素对小菜蛾Plutella xylostella (L.)进行了抗性选育，并对选育过程中小菜蛾解毒酶的活性进行了研究。选育从F₀至F₂₁代，抗性缓慢波动上升，达到选育前的122.91倍；F₂₁至F₂₇代，抗性迅速增长，达到选育前的812.73倍，抗性发展趋势呈现S型曲线。随着选育代数的增加，对乙酰胆碱酯酶(AChE)没有明显的影响；羧酸酯酶(CarE)活性,F₂₇是F₀的1.5倍，从F₂₂开始，活性在较高水平上波动；谷胱甘肽转移酶(GST)活性F₂₇是F₀的2.2倍，且从F₁₈开始，活性在较高水平上波动。选育的抗性品系，增效醚对阿维菌素增效6.34倍。

关键词: 小菜蛾, 阿维菌素, 抗性选育, 解毒酶, 乙酰胆碱酯酶

Abstract: The resistance selection of diamondback moth (DBM), Plutella xylostella (L.), to avermectins (AVMs) and its effect on activities of detoxification enzymes in AVMsselected DBM were investigated during 1996～1999. After 27 generations of continuous selection pressure, resistance to AVMs was over 812-folds in this strain compared with the unselected parents. Resistance selection by AVMs has no effect on activity of acetylcholinesterase (AChE). The activities of carboxylesterase (CarE) and glutathione S-transferases (GST) in DBM larvae of F₂₇ generation increased 1.5 and 2.2-folds respectively, compared with the unselected parents (F₀). Piperonyl butoxide (Pb) can moderately increase the toxicity of AVMs to the selected strain for about 6-folds. It suggests that the resistant mechanisms of DBM to AVMs were probably associated with microsomal mixed function oxidases (MFO), GST and CarE.

Key words: Plutella xylostella, avermectins, resistance selection, detoxification, acetylcholinesterase

李腾武，高希武，郑炳宗，许向丽. 阿维菌素对小菜蛾的抗性选育及其对解毒酶活性的影响[J]. , 2000, 43(-1): 38-43.

LI Teng-wu, GAO Xi-wu, ZHENG Bing-zong, XU Xiang-li. Author for correspondenceStudy on resistance selection by avermectins and its effect onactivities of detoxification enzymes inPlutella xylostella(L.)[J]. , 2000, 43(-1): 38-43.

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