›› 2000, Vol. 43 ›› Issue (-1): 85-91.

• 研究论文 • 上一篇    下一篇

苏云金杆菌δ-内毒素、芽孢及苏云金素A对棉铃虫毒性及拒食性的比较

张继红,王琛柱   

  • 出版日期:2000-12-20 发布日期:2000-12-20

Comparison of toxicity and deterrence among crystal, spore and thuringiensin A of Bacillus thuringiensis against Helicoverpa armigera (Hübner)

ZHANG Ji-hong, WANG Chen-zhu   

  • Online:2000-12-20 Published:2000-12-20
  • Contact: WANG Chen-zhu

摘要: 苏云金杆菌库斯塔克变种HD-1的晶体蛋白与芽孢、HD-1无晶体突变株(Cry-)的芽孢以及苏云金素A对棉铃虫Helicoverpa armigera毒性和拒食性的比较研究显示,HD-1晶体蛋白对棉铃虫的杀虫毒力高, 拒食作用强; HD-1芽孢对棉铃虫具有一定的杀虫活性和生长抑制作用, 并有很强的拒食作用; HD-1无晶体突变株(Cry-)芽孢对棉铃虫无毒也无拒食作用;苏云金素A对棉铃虫的生长发育有极显著的抑制作用, 但对棉铃虫无拒食作用,由此证明晶体蛋白是苏云金杆菌杀虫活性和拒食作用的主要来源。苏云金素A与苏云金杆菌芽孢晶体混合物一起使用, 可使棉铃虫的死亡率显著提高。

关键词: 棉铃虫, 苏云金杆菌, 毒性, 拒食性

Abstract: A comparative study on the toxicity and deterrence among crystal, spore and thuringiensin A of Bacillus thuringiensis against Helicoverpa armigera(Hübner) was carried out. The parasporal crystals of Btk HD-1 were highly toxic and feeding deterrent to H. armigera larvae. The spores of Btk HD-1 showed some insecticidal activity and highly feeding deterrence. The spores of a HD-1 mutant without crystal, Cry-, had no toxicity and feeding deterrence. The thuringiensin A greatly inhibited larval development, but had no feeding deterrence. In the no choice test, larval feeding was arrested by HD-1 crystals, but HD-1 spores, mutant Cry- spores and thuringiensin A had no effect on larval feeding during the 45 min observation. When the crystal-spore mixture is combined with thuringiensin A, larval mortality was greatly increased.

Key words: Helicoverpa armigera, Bacillus thuringiensis, toxicity, deterrence