棉花抗虫工程菌对棉铃虫的离体及活体生物测定

摘要/Abstract

摘要： 棉花抗虫工程菌是以能在棉株体内定殖的优势内生细菌Bacillus cereus (Bc9102)为宿主菌，将Bt kurstaki的δ-内毒素基因cryIA©整合到其染色体上形成的内生工程菌。以棉铃虫Helicoverpa armigera为供试昆虫的离体生测结果表明：在同一剂量水平上，HE-1、HE-2、ME14-2、ME14-3、MK14-1等工程菌株对棉铃虫幼虫的毒力高于或等于Bt野生菌株HD-73，浓度最高时这几个工程菌处理的棉铃虫死亡率分别为96.7%、83.3%、93.3%、83.3%、80.0%，而HD-73为80.0%，死亡率与浓度呈正相关。在用上述工程菌接种棉花的活体生测中，注射处理和喷雾处理法杀虫效果均优于浸种处理法。以HE-1为例，注射、喷雾、浸种处理4周后棉铃虫校正死亡率分别达到90.0%、76.0%和23.3%。工程菌对棉铃虫的生长发育也有抑制作用。

关键词: 蜡质芽孢杆菌, 抗虫工程菌, 棉铃虫

Abstract: Insecticidal engineered bacteria of cotton are recombinant bacteria by introducing the cryIA© gene from Bacillus thuringiensis subsp. Kurstaki into the chromosome of Bacillus cereus(Bc9102) which is a dominant endophytic bacterium in cotton. In vitro bioassays with cotton bollworm (Helicoverpa armigera) was conducted and the results showed: at each concentration level, engineered strains HE-1,HE-2,ME14-2,ME14-3 and MK14-1 had a higher insecticidal activity than Bt strain HD-73, causing 96.7%,83.3%,93.3%,83.3%,80.0% mortality respectively at the highest concentration while Bt HD-73 had 80% mortality. The concentration mortalitey curves of these strains showed a linear correlation. In planta bioassays, the results showed that insecticidal activity of cotton injected or sprayed with engineered bacteria was obviously better than that of cotton seedtreated with engineered bacteria. For example, engineered strain HE-1 had larval mortality respectively 90.0%、76.0% and 23.3% after injection,spraying and seedtreatment 4 weeks. At the same time, engineered bacteria restrained the development of cotton bollworms.

Key words: Bacillus cereus, insecticidal engineered bacteria, cotton bollworms

徐静，寻广新，赵同海，张青文，周明爿羊. 棉花抗虫工程菌对棉铃虫的离体及活体生物测定[J]. , 2000, 43(-1): 92-97.

XU Jing, XUN Guang-xin, ZHAO Tong-hai, ZHANG Qing-wen, CHEO Ming-tang. In vitro and planta bioassay of engineered endophyticbacteria in cotton with cotton bollworms[J]. , 2000, 43(-1): 92-97.

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棉花抗虫工程菌对棉铃虫的离体及活体生物测定

In vitro and planta bioassay of engineered endophyticbacteria in cotton with cotton bollworms

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