›› 2000, Vol. 43 ›› Issue (3): 285-290.

• 研究论文 • 上一篇    下一篇

粘虫咽侧体静止激素的初步分离纯化

欧阳迎春,唐爽,关雪辰,方宇凌   

  • 出版日期:2000-08-20 发布日期:2000-08-20

Preliminary isolation and purification of allatostatin from Mythimnaseparata

OUYANG Ying-chun,TANG Shuang,GUAN Xue-chen, FANG Yu-ling   

  • Online:2000-08-20 Published:2000-08-20

摘要: 用放射化学的方法,检测粘虫Mythimna separata幼虫脑提取物中咽侧体静止激素(Allatostatin,AS)样的活性物质。发现粘虫幼虫脑中含有AS样的活性物质,可抑制离体咽侧体(Corporaallata,CA)的保幼激素(Juvenile hormone,JH)的生物合成。用1个脑当量的幼虫脑提取物,对CA的JH合成的抑制率达51%。幼虫脑提取物经胰蛋白酶水解后,AS活性显著降低,表明幼虫脑中的活性物质是肽类或蛋白质类物质。幼虫脑提取物用Sep-Pak柱初步纯化,有活性的组分经高压液相色谱(HPLC)分离,洗脱组分的AS活性测定表明,有AS活性的组分主要集中在组分1~20和组分30~60,其中对离体CA的JH合成的抑制大于50%的组分有3、5、11、40、54和60。

关键词: 粘虫, 咽侧体静止激素, 脑提取物, 高压液相色谱

Abstract: In this report, a preliminary isolation of AS from the brains of larvae, Mythimna separata, is described. The ASactivity material which can inhibit JH synthesis by CA occurs in the brains of last instar. The ability of brain extract to inhibit JH synthesis in vitro was tested by radiochemical assay. Using 1 brain equivalent, 51% inhibition of JH synthesis was achieved. If the brain extract was pretreated with trypsin, the inhibitory effect of the extract was greatly reduced. Assays of fractions from the reversalphase C18 column of HPLC separation showed that materials with AS activity were eluted between 5.2% to 28% methanol (fraction 1~20), and between 40% to 76% methanol (fraction 30~60). Assays of fractions 3, 5, 11, 40, 54 and 60 using 5 brain equivalents per pair of CA showed >50% inhibition of JH synthesis. Further purification of the fractions from the C18 column with allatostatic activity is in progress.

Key words: Mythimna separata, allatostatin, brain extract, HPLC