唐古特瑞香提取物对菜粉蝶幼虫的毒杀作用

›› 2000, Vol. 43 ›› Issue (4): 364-372.

唐古特瑞香提取物对菜粉蝶幼虫的毒杀作用

徐汉虹¹，陈立¹，赵善欢¹，孙洪发²，纪兰菊²

出版日期:2000-11-20 发布日期:2000-11-20

Insecticidal activities of Daphne tangutica extracts on Pieris rapae larvae

XU Han-hong¹, CHEN Li¹, ZHAO Shan-huan¹, SUN Hong-fa², JI Lan-ju²

Online:2000-11-20 Published:2000-11-20

摘要/Abstract

摘要： 生测表明，唐古特瑞香Daphne tangutica的甲醇抽提物具有明显的拒食和胃毒活性，活性跟踪得到流分9。流分9导致菜粉蝶Pieris rapae幼虫体小，中肠组织细胞受到破坏，脂肪体呈消融状；对试虫生理指标的影响主要表现在降低血淋巴蛋白质含量和抑制中肠酯酶活力。

关键词: 唐古特瑞香, 菜粉蝶, 生物活性, 生理效应

Abstract: Bioassays of the methanol extract of Daphne tangutica, a local endemic plant in QinghaiTebet Plateau, for insecticidal effects on the imported cabbage worm, Pieris rapae, were carried out. The results showed that the extract possessed high effects of antifeedant and stomach poison. Fraction 9 of Silica gel column chromatography proved to be the most active fraction among those partitioned from the methanol extract by bioassaydriven separation. The larvae fed on leaf discs treated with fraction 9 grew slowly, and their bodies were smaller than those of the control. Histological study indicated that the tissue of midgut was destroyed and fatbody became to fade out by autolysis. The preliminary physiological reaction studies showed that fraction 9 could reduce the content of protein in haemolymph and inhibited the activity of esterase in midgut significantly.

Key words: Daphne tangutica, Pieris rapae, bioactivity, physiological reaction

徐汉虹¹，陈立¹，赵善欢¹，孙洪发²，纪兰菊². 唐古特瑞香提取物对菜粉蝶幼虫的毒杀作用[J]. , 2000, 43(4): 364-372.

XU Han-hong¹, CHEN Li¹, ZHAO Shan-huan¹, SUN Hong-fa², JI Lan-ju². Insecticidal activities of Daphne tangutica extracts on Pieris rapae larvae[J]. , 2000, 43(4): 364-372.

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