长角血蜱若虫发育期20-羟基蜕皮酮含量变化与表皮发生的关系

摘要/Abstract

摘要： 用高效液相色谱和放射免疫分析对长角血蜱Haemaphysalis longicornis若虫发育期整体蜕皮激素(20-E)含量变化进行了测定，并用扫描电镜对其表皮结构及其发生进行了观察。20-E存在于若虫整个发育期，饥饿、吸血和饱血后前4天，激素水平低(0.71～1.30 ng/头)；饱血后6天明显增加，此时皮层溶离开始，蜕皮间隙形成；饱血后7天继续增加；饱血后8天，20-E含量达到高峰(8.70 ng/头)，此时若虫开始沉积新的上表皮；高峰后，20-E含量急剧下降，直到蜕皮前保持低水平，此期内沉积新的原表皮，旧表皮被部分吸收。上述结果显示出20-E含量急剧增加及其高峰分别与启动皮层溶离和新的上表皮沉积相吻合，20-E与新的原表皮沉积无关。

关键词: 长角血蜱, 若虫, 20羟基蜕皮酮, 表皮发生

Abstract: The changes of ecdysteroid (20-E) in the whole body and cuticulogenesis during nymphal stage were studied by HPLC and RIA, and SEM respectively. 20-E existed in all the nymphal stages. Its level was low in nymphs unfed, feeding and during the first 4 days after engorgement (0.71～1.30ng/nymph). Then it began to increase sharply on the 6th day after engorgement when apolysis was initiated and exuvial space appeared. 20-E reached its peak (8.70 ng/nymph) during the deposition of the new epicuticle on the 8th day after engorgement. Thereafter, 20-E dropped to low value again. The procuticle deposition and old cuticle digestion occurred during this period. There results demonstrated that the highest concentration and sharp increase of 20-E were coincident with the deposition of new epicuticle and the formation of exuvial space (apolysis) separately. No relationship was observed between 20-E concentration and new procuticle synthesis.

Key words: Haemaphysalis longicornis, nymph, 20-hydroxyecdysone, cuticulogenesis

刘敬泽¹，李永良²，姜在阶². 长角血蜱若虫发育期20-羟基蜕皮酮含量变化与表皮发生的关系[J]. , 2001, 44(1): 46-50.

LIU Jing-ze¹, LI Yong-liang², JIANG Zai-jie². Correlation between 20-hydroxyecdysone levels and cuticulogenesisin nymphs of Haemaphysalis longicornis[J]. , 2001, 44(1): 46-50.

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长角血蜱若虫发育期20-羟基蜕皮酮含量变化与表皮发生的关系

Correlation between 20-hydroxyecdysone levels and cuticulogenesisin nymphs of Haemaphysalis longicornis

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