棉铃虫不同发育阶段微粒体P450酶系组成和活性的比较

›› 2001, Vol. 44 ›› Issue (2): 142-147.

棉铃虫不同发育阶段微粒体P450酶系组成和活性的比较

邱星辉，李薇，冷欣夫

出版日期:2001-05-20 发布日期:2001-05-20

Comparison of components and activities of microsomal P450 monooxygenases from the cotton bollworm,Helicoverpa armigera during different developmental stages

QIU Xing-hui, LI Wei, LENG Xin-fu

Online:2001-05-20 Published:2001-05-20

摘要/Abstract

摘要： 比较了棉铃虫Helicoverpa armigera 6龄幼虫、蛹、成虫微粒体P450单加氧酶系组成及其活性。P450含量在6龄幼虫中肠>（脂肪体=蛹）>成虫，NADPH-细胞色素还原酶在幼虫中肠>幼虫脂肪体>蛹>成虫；6龄幼虫脂肪体微粒体与蛹脂肪体微粒体P450含量相近，但NADPH-细胞色素还原酶活性前者是后者的4.2倍；成虫微粒体的细胞色素P450和NADPH细胞色素P450还原酶含量很低，几乎未检测出。用对-硝基苯甲醚和艾氏剂为底物测定P450酶系活性表明，与6龄幼虫相比，蛹和成虫具有极低的单加氧酶活性，其O-脱甲基酶活性未检出，艾氏剂环氧化酶活性比幼虫低2～3个数量级。

关键词: 棉铃虫, P450单加氧酶, 生长发育表达

Abstract: The components and activities of microsomal P450 monooxygenases in 6th instar larva, pupa and adult were examined.The results showed that the levels of P450 and NADPH-P450 reductase were in the order of 6th instar larval midgut>pupae>adults. The contents of cytochrome P450 in pupa was similar to that in 6th instar larval fatbody,while the level of NADPH-cytochrome P450 reductase in the former was only about quarter as much as that in the latter.In adult microsome, the two components were undetectable.Compared with 6th instar larvae,the assayed activities were much lower in adult and pupa: the pnitroanisole demethylation was undetectable and aldrin epoxidation was 10 fold as that in 6th instar larva.

Key words: Helicoverpa armigera, cytochrome P450 monooxygenase, developmental expression

邱星辉，李薇，冷欣夫. 棉铃虫不同发育阶段微粒体P450酶系组成和活性的比较[J]. , 2001, 44(2): 142-147.

QIU Xing-hui, LI Wei, LENG Xin-fu. Comparison of components and activities of microsomal P450 monooxygenases from the cotton bollworm,Helicoverpa armigera during different developmental stages[J]. , 2001, 44(2): 142-147.

[1]	艾东, 林荣华, 王猛, 梁晓贺, 于彩虹. 棉铃虫可溶型海藻糖酶的化学修饰[J]. , 2018, 61(7): 801-807.
[2]	李兆群, 张帅, 周淑芬, 雒珺瑜, 崔金杰. 棉铃虫气味结合蛋白HarmOBP16的组织表达谱及配基结合特性分析[J]. , 2017, 60(8): 891-899.
[3]	王芹芹, 崔丽, 王奇渊, 杨宏宇, 芮昌辉. 棉铃虫对茚虫威的抗性机理: PBO, DEF和DEM的增效作用及解毒酶活性[J]. , 2017, 60(8): 912-919.
[4]	公玲玲, 张丹丹, 郑曰英, 于佃平, 肖玉涛, 曲爱军. 棉铃虫ABC转运蛋白ABCG1的基因克隆、亚细胞定位及其与Cry1Ac毒力的关系[J]. , 2017, 60(3): 297-308.
[5]	王冰洁, 袁向东, 赵曼, 刘臣, 陈琳, 梁革梅. 棉铃虫中肠钙粘蛋白、氨肽酶N及碱性磷酸酯酶的抗血清对Cry1Ac和Cry2Aa毒力的影响[J]. , 2016, 59(9): 977-984.
[6]	于彩虹, 艾东, 梁晓贺, 王振波, 郭建军, 姜辉, 林荣华. 栗精碱对棉铃虫中肠组织可溶型海藻糖酶的抑制效应[J]. , 2016, 59(3): 301-308.
[7]	张帅, 马艳, 闵红, 于晓庆, 李娜, 芮昌辉, 高希武. 华北棉区主要害虫抗药性监测与治理技术示范[J]. , 2016, 59(11): 1238-1245.
[8]	董钧锋, 宋月芹, 孙九光, 闫志宁, 孙亚兰, 王琛柱. 棉铃虫齿唇姬蜂嗅觉受体基因 CchlOrco 的克隆及组织表达谱分析[J]. , 2015, 58(6): 603-609.
[9]	石庆型, 罗庆怀, 赵龙, 周正湘, 何广全, 韦卫. 与中红侧沟茧蜂生境与寄主定位相关的玉米及棉铃虫幼虫体表挥发性成分的提取与鉴定[J]. , 2015, 58(3): 244-255.
[10]	陈伟, 徐卫华. 棉铃虫转录因子c-Myc基因在滞育和非滞育蛹脑中的表达分析及多克隆抗体制备[J]. , 2015, 58(2): 115-121.
[11]	邓盼, 马伟华, 李国清. 棉铃虫幼虫自相残杀习性与其龄期和食物营养的相关性（英文）[J]. , 2015, 58(2): 175-180.
[12]	陈伟, 徐卫华. 棉铃虫Wnt-1基因克隆、多克隆抗体制备及在滞育和非滞育蛹脑中的表达检测[J]. , 2015, 58(1): 15-21.
[13]	曹婧, 欧阳芳, 戈峰, 杨中侠, 肖铁光. 取食Bt棉与玉米的越冬代棉铃虫抗寒性特征比较[J]. , 2015, 58(1): 45-52.
[14]	闫硕，李慧婷，朱威龙，朱家林，张青文，刘小侠. 光强度对棉铃虫交配行为的影响[J]. , 2014, 57(9): 1045-1050.
[15]	刘宁灿，张进，王桂荣，刘杨，严善春. 棉铃虫普通气味受体基因HarmOR9和HarmOR29的克隆和组织表达分析[J]. , 2014, 57(5): 522-529.