粘虫颗粒体病毒增效因子的基因定位

›› 2001, Vol. 44 ›› Issue (2): 148-154.

粘虫颗粒体病毒增效因子的基因定位

刘强¹，叶寅²，白小东¹，丁翠^1*

出版日期:2001-05-20 发布日期:2001-05-20

Location of the gene encoding synergistic factorof the Pseudaletia unipuncta granulosis virus

LIU Qiang¹, YE Yin², BAI Xiao-dong¹, DING Cui¹

Online:2001-05-20 Published:2001-05-20

摘要/Abstract

摘要： 参考粉纹夜蛾Trichoplusia ni 颗粒体病毒增强因子的基因序列，设计PCR引物，用PCR反应扩增出特异性产物。用EcoRⅠ、BamHⅠ双酶酶切处理PCR反应产物，然后克隆到质粒pUC19中，构建重组质粒pUC19-SF；对重组质粒pUC19-SF中的外源片段测序，结果证明PCR扩增产物是粘虫颗粒体病毒PuGV-Ps增效因子基因的一段序列。重组质粒pUC19-SF的插入片段标记为探针，通过Southern杂交将增效因子基因定位于PuGV-Ps病毒基因组的多种酶切片段上。

关键词: 粘虫颗粒体病毒, 增效因子, 基因定位, Southern杂交

Abstract: Using the nucleotide sequence of the VEF (viral enhancing factor) gene of Trichoplusia ni granulosis virus for reference, we designed a primer for PCR and amplified a specific fragment by PCR reaction. A recombinant plasmid pUC19-SF was constructed by cloning the PCR product which was treated with EcoRⅠ, BamHⅠ into pUC19. The PCR product was proven to be a fragment of the gene encoding the synergistic factor of Pseudaletia unipuncta granulosis virus (PuGV-Ps) by DNA sequencing. After labeling this fragment as a probe, the gene encoding of the synergistic factor was located in many restrictive fragments of PuGV-Ps genome by Southern blot hybridization.

Key words: Pseudaletia unipuncta granulosis virus, synergistic factor, genetic location, Southern blot hybridization

刘强¹，叶寅²，白小东¹，丁翠^1*. 粘虫颗粒体病毒增效因子的基因定位[J]. , 2001, 44(2): 148-154.

LIU Qiang¹, YE Yin², BAI Xiao-dong¹, DING Cui¹. Location of the gene encoding synergistic factorof the Pseudaletia unipuncta granulosis virus[J]. , 2001, 44(2): 148-154.

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