›› 2001, Vol. 44 ›› Issue (4): 494-500.

• 研究论文 • 上一篇    下一篇

长蠹科几种检疫性害虫的ND4基因序列及系统进化

黄永成1, 李伟丰1, 陆温2, 陈永久3, 张亚平3   

  • 出版日期:2001-11-20 发布日期:2001-11-20

Mitochondrial DNA ND4 sequence variation and phylogeny of five species of Bostrychidae (Coleoptera)

HUANG Yong-cheng1, LI Wei-feng1, LU Wen2, CHEN Yong-jiu3,ZHANG Ya-ping3   

  • Online:2001-11-20 Published:2001-11-20

摘要: 长蠹科昆虫严重危害林木和仓贮物品。应用非损伤性DNA测序技术测定了来自不同国家的长蠹科害虫的线粒体DNA ND4 基因的部分序列。在获得的204 bp的序列中,5种昆虫的序列变异丰富,多数变异发生在密码子的第3位点上。用PAUP3.1.1数据分析软件构建了5个种的合意简约树。并将实验结果与形态学特征比较分析,探讨5个种及所在属的系统进化。结果表明:双钩异翅长蠹所在的异翅长蠹属分化最早,其次是竹大长蠹所在的大长蠹属、双棘长蠹和黑双棘长蠹所在的双棘长蠹属及红艳长蠹所在的钻木长蠹属。双棘长蠹和黑双棘长蠹隶属同一个属,遗传关系最近,分化最晚,与形态学研究结果相吻合。

关键词: 长蠹科, ND4基因, mtDNA, 系统进化

Abstract: The Bostrychidae represent a highly destructive group of wood-boring and stored product beetles. We sequenced the mitochondrioal DNA gene encoding NADH dehydrogenase subunit 4(ND4) with non-invasive DNA Genotyping from 7 samples of 5 species of this family. The length of sequences was 204 bp. Sequencecomparison analysis indicated high variations in sequences of each species. The ND4 phylogenetic trees had been constructed for 5 Bostrychidae species with PAUP 3.1.1. This result was compared with the results based on the morphology of 5 species. Furthermore, the phylogenetic relationship among the species and genera that each species belongs to were discussed. The results indicated that H. aequalis of genus Heterobostrychus diverged the earliest, then did B. parallela of genus Bostrychopsis, S. anale and S.conigerum of genus Sinoxylon, X. religiosus of genus Xylothrips in turns. The two species of S. anale and S. conigerum were closely related, and they diverged last. This was basically identical with the results based on morphology.

Key words: ND4, mtDNA, phylogeny, Bostrychidae