›› 2002, Vol. 45 ›› Issue (1): 58-62.

• 研究论文 • 上一篇    下一篇

桔全爪螨的抗药性选育及其解毒酶活力变化

孟和生, 王开运, 姜兴印, 仪美芹   

  • 出版日期:2002-02-20 发布日期:2002-02-20

Studies on resistance selection by abamectin and fenpropathrin and activity change of detoxicant enzymes in Panonychus citri

MENG He-Sheng, WANG Kai-Yun, JIANG Xing-Yin, YI Mei-Qin   

  • Online:2002-02-20 Published:2002-02-20

摘要: 在室内模拟田间药剂的选择压力,用齐墩螨素和甲氰菊酯对桔全爪螨Panonychus citri 逐代处理,以选育其抗药性种群。选育至12代,对齐墩螨素抗性增长到7.30倍,对甲氰菊酯抗性增长到17.11倍。增效剂和离体酶活性的测定结果表明,抗性种群的乙酰胆碱酯酶和多功能氧化酶的活性都提高了;推测羧酸酯酶、乙酰胆碱酯酶、多功能氧化酶和谷胱甘肽-S-转移酶比活力的增加是桔全爪螨对甲氰菊酯产生抗性的主要原因。

关键词: 桔全爪螨, 齐墩螨素, 甲氰菊酯, 抗性选育, 解毒酶

Abstract: Studies on resistance selection by abamectin and fenpropathrin and activity change of detoxicant enzymes of Panonychus citri(McGregor)were carried out in laboratory. P. citri was separately treated with abamectin and fenpropathrin imitating field chemicals selection pressure in order to breed its resistance. The results showed that the resistance has respectively reached 7.30-fold to abamectin and 17.11-fold to fenpropathrin after 12 times selection. The resistance mechanism was evaluated by activity measurement of enzymic inhibitors and detoxicant enzymes. It was concluded that the rise of resistance to abamectin was mainly associated with the increasing activities of acetylcholinesterase and mixedfunction oxidases. The resistance to fenpropathrin was attributed to carboxylesterase, acetylcholinesterase and glutathione transferase.

Key words: Panonychus citri, abamectin, fenpropathrin, resistanceselection, detoxicant enzymes