昆虫学报 ›› 2002, Vol. 45 ›› Issue (2): 170-174.

• 研究论文 • 上一篇    下一篇

蝗虫微孢子虫对东亚飞蝗卵黄原蛋白含量的影响

陈建新,沈杰,宋敦伦,张龙,严毓骅   

  • 出版日期:2002-04-20 发布日期:2002-04-20

Effect of Nosema locustae on the content of vitellogenin of Locusta migratoria manilensis

CHEN Jian-Xin, SHEN Jie, SONG Dun-Lun, ZHANG Long, YAN Yu-Hua   

  • Online:2002-04-20 Published:2002-04-20

摘要: 采用免疫学方法,对东亚飞蝗Locusta migratoria manilensis感染蝗虫微孢子虫Nosema locustae后体内卵黄蛋白含量的变化进行了研究。结果表明,感病蝗虫与对照健虫相比,卵黄发生有严重障碍,脂肪体和卵巢中卵黄原蛋白或卵黄蛋白含量极低,导致感病雌虫丧失产卵能力。脂肪体中卵黄原蛋白含量最高峰健虫为18.7 mg/mL,而病虫只有4.7 mg/mL;血淋巴中卵黄原蛋白含量最高峰健虫为7.6 mg/mL,而病虫只有2.6 mg/mL;卵巢中卵黄蛋白含量最高峰健虫为73.4 mg/mL,而病虫只有4.9 mg/mL。

关键词: 东亚飞蝗, 蝗虫微孢子虫, 卵黄发生, 卵黄原蛋白, 卵黄蛋白

Abstract: Changes in the content of vitellogenin in Locusta migratoria manilensis infected with Nosema locustae were determined by immunology method. The results indicated that vitellogenin did not accomplish in the locust infected, and the contents of vitellogenin in fat body, haemolymph and ovary were very low compared with the control. The maximum content of vitellogenin in fat body in the treatment was 4.663 mg/mL, while the value was 18.655 mg/mL in the control. The maximum content of vitellogenin in haemolymph in the treatment was 2.627 mg/mL, while the value was 7.603 mg/mL in the control. The maximum content of vitellogenin in ovary in the treatment was 4.927 mg/mL while the value was 73.367 mg/mL in the control.

Key words: Locusta migratoria manilensis; Nosema locustae, vitellogenesis, vitellogenin, vitellin