关键词: 中国野桑蚕, RAPD分析, 分子系统树, 遗传距离

Abstract: The random amplified polymorphism DNA (RAPD) analysis of the mulberry wild silkworm, Bombyx mandarina, from 11 representative regions in China was made. The results showed that the genetic distance of the mulberry wild silkworm in different regions was quite high, from 0.465(Ankang-Zhenjiang) to 0.209(Wuhan-Hefei); the genetic distance between individuals of the mulberry wild silkworm from the same region was also high, from 0.144 to 0.318; both were higher than the genetic distance between individuals of the silkworm, Bombyx mori, and even higher than the genetic distance between varieties of the silkworm. These results suggested that the mulberry wild silkworm from China be a very complex population with abundant genetic diversity. Furthermore the genetic distance of the mulberry wild silkworm was positively related to the distance of space in most cases. The positions of the mulberry wild silkworm from Shaanxi were very peculiar in the molecular dendrogram,suggesting the importance of this region in the evolution and diversification of this specics.

Key words: Bombyx mandarina, RAPD analysis, molecular dendrogram, genetic distance