›› 2002, Vol. 45 ›› Issue (3): 290-295.

• 研究论文 • 上一篇    下一篇

家蚕微粒子病原虫(Nosema bombycis)小亚基核糖体RNA全基因的克隆及其二级结构的构建

王见杨1, 黄可威1, 陆长德2   

  • 出版日期:2002-06-20 发布日期:2002-06-20

Analyses of small subunit ribosomal RNA sequence of the microsporidium, Nosema bombycis and its secondary structure

WANG Jian-Yang1, HUANG Ke-Wei1, LU Chang-De2   

  • Online:2002-06-20 Published:2002-06-20

摘要: 在用PCR技术扩增、克隆、测序了家蚕微粒子病原虫Nosema bombycis (镇江株)小亚基核糖体RNA基因核心序列(5'-端起1 200 bp)的基础上,用SSP-PCR技术克隆了核心序列3'-端下游序列,从而获得了家蚕微粒子病原虫小亚基核糖体RNA基因的全序列共1 233 bp。 用RnaViz 、Forcon、DCSE等生物软件构建了家蚕微粒子病原虫小亚基核糖体RNA的二级结构,与其它微孢子虫及真核生物小亚基核糖体RNA的二级结构相比,该二级结构缺乏螺旋10、E10-1、11、18、E23-n和43。

关键词: 家蚕微粒子病原虫, SSP-PCR, 小亚基核糖体RNA(SSUrRNA)全基因, 二级结构, 螺旋

Abstract: The nucleotide sequence (1 205 bp) of small subunit ribosomal RNA (SSUrRNA) of the microsporidium, Nosema bombycis (Zhenjiang), was amplified by polymerase chain reaction(PCR). Its 3'-end was obtained using the single specific primer-PCR technique with the primers matching the highly conserved parts of SSUrRNA genes cloned. The entire coding region for SSUrRNA of N. bombycis (Zhenjiang) was 1 233 bp as described elsewhere. The sequence shared high homology with those of many microsporidia, especially N. apis. The secondary structure of the SSUrRNA was constructed with RnaViz, Forcon and DCSE. There were no helices such as 10, E10-1, 11,18, E23-n and 43 in it.

Key words: Nosema bombycis, SSP-PCR, SSUrRNA gene, secondary structure, helix